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Glycosaminoglycan mimetic improves enrichment and cell functions of human endothelial progenitor cell colonies.
Chevalier F, Lavergne M, Negroni E, Ferratge S, Carpentier G, Gilbert-Sirieix M, Siñeriz F, Uzan G, Albanese P. Chevalier F, et al. Stem Cell Res. 2014 May;12(3):703-15. doi: 10.1016/j.scr.2014.03.001. Epub 2014 Mar 10. Stem Cell Res. 2014. PMID: 24681520 Free article.
Human circulating endothelial progenitor cells isolated from peripheral blood generate in culture cells with features of endothelial cells named late-outgrowth endothelial colony-forming cells (ECFC). In adult blood, ECFC display a constant quantitative and qualitative dec …
Human circulating endothelial progenitor cells isolated from peripheral blood generate in culture cells with features of endothelial cells n …
Generation of Genetically Modified Mice through the Microinjection of Oocytes.
Delerue F, Ittner LM. Delerue F, et al. J Vis Exp. 2017 Jun 15;(124):55765. doi: 10.3791/55765. J Vis Exp. 2017. PMID: 28654070 Free PMC article.
Together, the steps of the protocol will allow for the generation of genetically modified mice and for the subsequent establishment of mouse colonies for a plethora of research fields, including immunology, neuroscience, cancer, physiology, development, and others....
Together, the steps of the protocol will allow for the generation of genetically modified mice and for the subsequent establishment of mouse …
Multiplex PCR for rapid identification of major lactic acid bacteria genera in cider and other fermented foods.
Cousin FJ, Le Guellec R, Chuat V, Dalmasso M, Laplace JM, Cretenet M. Cousin FJ, et al. Int J Food Microbiol. 2019 Feb 16;291:17-24. doi: 10.1016/j.ijfoodmicro.2018.11.004. Epub 2018 Nov 6. Int J Food Microbiol. 2019. PMID: 30428422
This multiplex PCR worked equally with purified genomic DNA of bacterial isolates and with colonies directly picked from agar plates. This new PCR method was also successfully extended to wine and dairy isolates, and thus constitutes an effective tool to quickly identify L …
This multiplex PCR worked equally with purified genomic DNA of bacterial isolates and with colonies directly picked from agar plates. …
Prasinoderma singularis sp. nov. (Prasinophyceae, Chlorophyta), a solitary coccoid Prasinophyte from the South-East Pacific Ocean.
Jouenne F, Eikrem W, Le Gall F, Marie D, Johnsen G, Vaulot D. Jouenne F, et al. Protist. 2011 Jan;162(1):70-84. doi: 10.1016/j.protis.2010.04.005. Epub 2010 Jul 31. Protist. 2011. PMID: 20674490
When compared to P. coloniale, P. singularis sp. nov. is morphologically characterized by the absence of colonies, smaller cells with a thinner cell wall, and a second cell type with a different cell covering....
When compared to P. coloniale, P. singularis sp. nov. is morphologically characterized by the absence of colonies, smaller cells with …
Efficient Editing of the Nuclear APT Reporter Gene in Chlamydomonas reinhardtii via Expression of a CRISPR-Cas9 Module.
Guzmán-Zapata D, Sandoval-Vargas JM, Macedo-Osorio KS, Salgado-Manjarrez E, Castrejón-Flores JL, Oliver-Salvador MDC, Durán-Figueroa NV, Nogué F, Badillo-Corona JA. Guzmán-Zapata D, et al. Int J Mol Sci. 2019 Mar 12;20(5):1247. doi: 10.3390/ijms20051247. Int J Mol Sci. 2019. PMID: 30871076 Free PMC article.
Using sgRNAs targeting exons 1 and 3 of apt9, we obtained disruption efficiencies of 3 and 30% on preselected 2-FA resistant colonies, respectively. Our results show that transient expression of Cas9 and a sgRNA can be used for editing of the nuclear genome inexpensively a …
Using sgRNAs targeting exons 1 and 3 of apt9, we obtained disruption efficiencies of 3 and 30% on preselected 2-FA resistant colonies …
Blockade of bone morphogenetic protein signaling potentiates the pro-inflammatory phenotype induced by interleukin-17 and tumor necrosis factor-α combination in rheumatoid synoviocytes.
Varas A, Valencia J, Lavocat F, Martínez VG, Thiam NN, Hidalgo L, Fernández-Sevilla LM, Sacedón R, Vicente A, Miossec P. Varas A, et al. Arthritis Res Ther. 2015 Jul 28;17(1):192. doi: 10.1186/s13075-015-0710-6. Arthritis Res Ther. 2015. PMID: 26215036 Free PMC article.
Expression and production of pro-inflammatory cytokines (IL-6 and granulocyte-macrophage colony-stimulating factor), chemokines (IL-8, CCL2, CCL5, and CXCL10), and matrix metalloproteinases (MMP-1, -2, -3, -9, and -13) were analyzed. ...
Expression and production of pro-inflammatory cytokines (IL-6 and granulocyte-macrophage colony-stimulating factor), chemokines (IL-8 …
Effect of Brood Pheromone on Survival and Nutrient Intake of African Honey Bees (Apis mellifera scutellata) under Controlled Conditions.
Démares FJ, Yusuf AA, Nicolson SW, Pirk CWW. Démares FJ, et al. J Chem Ecol. 2017 May;43(5):443-450. doi: 10.1007/s10886-017-0840-1. Epub 2017 Apr 28. J Chem Ecol. 2017. PMID: 28455796
Brood pheromone (BP) is released by honey bee larvae to indicate their protein requirements to the colony. Although BP is known to modulate pollen and protein consumption, which in turn can affect physiological and morphological parameters, such as hypopharyngeal gland (HP …
Brood pheromone (BP) is released by honey bee larvae to indicate their protein requirements to the colony. Although BP is known to mo …