Nano-hydroxyapatite was synthesized by means of the hydrothermal treatment. The effects of nano-hydroxyapatite material on the behaviour of G2 liver cancer cells were explored. About 50% of cell viability was lost in nHAp material treated cells at 200 degrees C @ 5 h, followed by -30% in nHAp treated cells at 100 degrees C @ 5 h. Compared with control, nHAp material treated cells at 200 degrees C @ 5 h showed 60% and nHAp material treated cells at 100 degrees C @ 5 h showed 15% morphological change. Moreover, 50% of cell death was observed at 24 h incubation with nHAp material treated at 200 degrees C @ 5 h cells and 56% cell death at 48 h incubation and hence alters and disturbs the growth of cancer cells. In contrast, the nHAp material treated at 100 degrees C @ 5 h protects the cells and could be used for liver cancer cell treatment.