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In vitro calcified matrix deposition by human osteoblasts onto a zinc-containing bioactive glass.
Saino E, Grandi S, Quartarone E, Maliardi V, Galli D, Bloise N, Fassina L, De Angelis MG, Mustarelli P, Imbriani M, Visai L. Saino E, et al. Eur Cell Mater. 2011 Jan 14;21:59-72; discussion 72. doi: 10.22203/ecm.v021a05. Eur Cell Mater. 2011. PMID: 21240845 Free article.
Human Sarcoma Osteoblast (SAOS-2) cells were used to i) assess cytotoxicity by lactate dehydrogenase (LDH) release and ii) evaluate the deposition of a calcified extracellular matrix by ELISA assay and quantitative RT-PCR (qRT-PCR). In comparison with pure silica an …
Human Sarcoma Osteoblast (SAOS-2) cells were used to i) assess cytotoxicity by lactate dehydrogenase (LDH) release and ii) evaluate the depo …
The differentiation of human adipose-derived stem cells (hASCs) into osteoblasts is promoted by low amplitude, high frequency vibration treatment.
Prè D, Ceccarelli G, Gastaldi G, Asti A, Saino E, Visai L, Benazzo F, Cusella De Angelis MG, Magenes G. Prè D, et al. Bone. 2011 Aug;49(2):295-303. doi: 10.1016/j.bone.2011.04.013. Epub 2011 Apr 30. Bone. 2011. PMID: 21550433
The state of calcification of the extracellular matrix was determined using the alizarin assay, while the expression of extracellular matrix and associated mRNA was determined by ELISA assays and quantitative RT-PCR (qRT-PCR). ...
The state of calcification of the extracellular matrix was determined using the alizarin assay, while the expression of extracellular …
Stem Cells Grown in Osteogenic Medium on PLGA, PLGA/HA, and Titanium Scaffolds for Surgical Applications.
Asti A, Gastaldi G, Dorati R, Saino E, Conti B, Visai L, Benazzo F. Asti A, et al. Bioinorg Chem Appl. 2010;2010:831031. doi: 10.1155/2010/831031. Epub 2010 Dec 23. Bioinorg Chem Appl. 2010. PMID: 21234383 Free PMC article.
The aim of this study was to investigate the performance of PLGA and PLGA/HA scaffolds with a higher porosity, ranging between 75% and 84%, with respect to Ti scaffolds but with smaller pore size, seeded with hASCs to develop a model that could be used in the treatment of bone de …
The aim of this study was to investigate the performance of PLGA and PLGA/HA scaffolds with a higher porosity, ranging between 75% and 84%, …
An enzyme-linked immunosorbent assay system for quantitative determination of calphobindin I, a new placental anticoagulant protein, and its application to various specimens.
Nakao H, Nagoya T, Iwasaki A, Suda M, Hattori Y, Saino Y, Shidara Y, Maki M. Nakao H, et al. Chem Pharm Bull (Tokyo). 1990 Jul;38(7):1957-60. doi: 10.1248/cpb.38.1957. Chem Pharm Bull (Tokyo). 1990. PMID: 2148507
We developed a sandwich enzyme-linked immunosorbent assay (ELISA) system for calphobindin I (CPB-I), a new placental coagulation inhibitor, using two monoclonal antibodies. ...Using this ELISA system, CPB-I levels in many kinds of specime …
We developed a sandwich enzyme-linked immunosorbent assay (ELISA) system for calphobindin I (CPB-I), a ne …