Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My NCBI Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1979 1
1981 2
1982 2
1983 4
1984 9
1985 11
1986 5
1987 11
1988 7
1989 16
1990 16
1991 17
1992 15
1993 13
1994 19
1995 10
1996 10
1997 10
1998 15
1999 9
2000 13
2001 10
2002 9
2003 9
2004 12
2005 18
2006 12
2007 13
2008 17
2009 14
2010 23
2011 40
2012 41
2013 33
2014 58
2015 32
2016 49
2017 47
2018 49
2019 63
2020 59
2021 57
2022 78
2023 49
2024 17

Text availability

Article attribute

Article type

Publication date

Search Results

942 results

Results by year

Filters applied: . Clear all
Page 1
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
Mosmann T. Mosmann T. J Immunol Methods. 1983 Dec 16;65(1-2):55-63. doi: 10.1016/0022-1759(83)90303-4. J Immunol Methods. 1983. PMID: 6606682
A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells. ...The results c …
A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The a …
Enzyme-Linked Immunosorbent Assays.
Hornbeck PV. Hornbeck PV. Curr Protoc Immunol. 2015 Aug 3;110:2.1.1-2.1.23. doi: 10.1002/0471142735.im0201s110. Curr Protoc Immunol. 2015. PMID: 26237010
This unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell-surface antigens. ...One of the support protocols can be used to optimize the different ELISAs. A second support protocol presents a method for prepar …
This unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell-surface antigens. …
IGF2BP3 is an essential N6-methyladenosine biotarget for suppressing ferroptosis in lung adenocarcinoma cells.
Xu X, Cui J, Wang H, Ma L, Zhang X, Guo W, Xue X, Wang Y, Qiu S, Tian X, Miao Y, Wu M, Yu Y, Xu Y, Wang J, Qiao Y. Xu X, et al. Mater Today Bio. 2022 Nov 24;17:100503. doi: 10.1016/j.mtbio.2022.100503. eCollection 2022 Dec 15. Mater Today Bio. 2022. PMID: 36457846 Free PMC article.
Here, we found that insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3), an m(6)A reader protein, is highly expressed in LUAD and associated with poor prognosis. ...
Here, we found that insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3), an m(6)A reader protein, is highly expressed in LU …
Avidin-Biotin ELISA-Based Detection of 5hmC.
Olova NN. Olova NN. Methods Mol Biol. 2021;2272:65-76. doi: 10.1007/978-1-0716-1294-1_5. Methods Mol Biol. 2021. PMID: 34009609
The enzyme-linked immunosorbent assay (ELISA) technique has been developed half a century ago, and yet its role in molecular biology remains significant. ...This chapter provides the procedure of a highly reproducible indirect sandwich ELISA …
The enzyme-linked immunosorbent assay (ELISA) technique has been developed half a century ago, and yet it …
Optical Microfiber Reader for Enzyme-Linked Immunosorbent Assay.
Sun LP, Huang Y, Huang T, Yuan Z, Lin W, Sun Z, Yang M, Xiao P, Ma J, Wang W, Zhang Y, Liu Z, Guan BO. Sun LP, et al. Anal Chem. 2019 Nov 5;91(21):14141-14148. doi: 10.1021/acs.analchem.9b04119. Epub 2019 Oct 22. Anal Chem. 2019. PMID: 31599567
In clinical diagnosis, accurate and reliable measurement technologies for the detection of disease biomarkers at ultralow concentrations can provide guidance for the initiation of treatment and potentially improve survival for patients. Here, we demonstrate an optical microfiber …
In clinical diagnosis, accurate and reliable measurement technologies for the detection of disease biomarkers at ultralow concentrations can …
MultiSero: An Open-Source Multiplex-ELISA Platform for Measuring Antibody Responses to Infection.
Byrum JR, Waltari E, Janson O, Guo SM, Folkesson J, Chhun BB, Vinden J, Ivanov IE, Forst ML, Li H, Larson AG, Blackmon L, Liu Z, Wu W, Ahyong V, Tato CM, McCutcheon KM, Hoh R, Kelly JD, Martin JN, Peluso MJ, Henrich TJ, Deeks SG, Prakash M, Greenhouse B, Mehta SB, Pak JE. Byrum JR, et al. Pathogens. 2023 May 2;12(5):671. doi: 10.3390/pathogens12050671. Pathogens. 2023. PMID: 37242341 Free PMC article.
A multiplexed enzyme-linked immunosorbent assay (ELISA) that simultaneously measures antibody binding to multiple antigens can extend the impact of serosurveillance studies, particularly if the assay approaches the simplicity, robustness, …
A multiplexed enzyme-linked immunosorbent assay (ELISA) that simultaneously measures antibody binding to …
Coupling ELISA to smartphones for POCT of chronic and congenital Chagas disease.
Schaumburg F, Pujato N, Peverengo LM, Marcipar IS, Berli CLA. Schaumburg F, et al. Talanta. 2023 May 1;256:124246. doi: 10.1016/j.talanta.2022.124246. Epub 2023 Jan 5. Talanta. 2023. PMID: 36657239
The ELISA plate reader was replaced by smartphone camera and image processing, comprising algorithms to account for variability sources and spatial light non-uniformity; thus, additional hardware like a dark-box is not required. The agreement between samples classif …
The ELISA plate reader was replaced by smartphone camera and image processing, comprising algorithms to account for variabilit …
Histo-ELISA technique for quantification and localization of tissue components.
Li Z, Goebel S, Reimann A, Ungerer M. Li Z, et al. Sci Rep. 2020 Nov 16;10(1):19849. doi: 10.1038/s41598-020-76950-1. Sci Rep. 2020. PMID: 33199754 Free PMC article.
A novel Histo-ELISA technique is intended to facilitate quantification of target tissue proteins in a tissue section and involves the selection of target regions in the tissue section, application of streptavidin-conjugated HRP (horseradish peroxidase), coupled with peroxi …
A novel Histo-ELISA technique is intended to facilitate quantification of target tissue proteins in a tissue section and involves the …
Development of a microplate reader compatible microfluidic chip for ELISA.
Hou F, Zhang Q, Yang J, Li X, Yang X, Wang S, Cheng Z. Hou F, et al. Biomed Microdevices. 2012 Aug;14(4):729-37. doi: 10.1007/s10544-012-9654-7. Biomed Microdevices. 2012. PMID: 22526682
We report a novel microfluidic device use for sandwich enzyme-linked immunoassay assay (ELISA). The related procedures including the introduction of reagents, dilution and distribution of samples, as well as immobilization of enzyme can be readily carr …
We report a novel microfluidic device use for sandwich enzyme-linked immunoassay assay (ELISA). The related proc …
Development of rELISA using novel markers for the diagnosis of paratuberculosis.
Jain M, Kumar A, Polavarapu R, Gupta S, Aseri GK, Sharma D, Sohal JS. Jain M, et al. J Immunol Methods. 2021 Oct;497:113105. doi: 10.1016/j.jim.2021.113105. Epub 2021 Jul 21. J Immunol Methods. 2021. PMID: 34298067
The assay was first optimized using traditional ELISA reader and then the performance was evaluated using a handheld ELISA reader. Findings were identical in both traditional ELISA reader as well as handheld ELISA reader …
The assay was first optimized using traditional ELISA reader and then the performance was evaluated using a handheld …
942 results