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Toll-like receptor 8 agonists improve NK-cell function primarily targeting CD56brightCD16- subset.
Veneziani I, Alicata C, Pelosi A, Landolina N, Ricci B, D'Oria V, Fagotti A, Scambia G, Moretta L, Maggi E. Veneziani I, et al. J Immunother Cancer. 2022 Jan;10(1):e003385. doi: 10.1136/jitc-2021-003385. J Immunother Cancer. 2022. PMID: 35091452 Free PMC article.
In some experiments, we purified CD56(bright)CD16(-) and CD56(dim)CD16(+) cells subsets by using NK Cell Isolation Kit Activation marker, cytokine production, CD107a expression and cytotoxicity assay were evaluated by flow cytometry. Cytokine release was quantified by E …
In some experiments, we purified CD56(bright)CD16(-) and CD56(dim)CD16(+) cells subsets by using NK Cell Isolation Kit Activation marker, cy …
Lack of Evidence of Chikungunya Virus Infection among Blood Donors during the Chikungunya Outbreak in Lazio Region, Italy, 2017.
Venturi G, Fabiani M, Amendola A, Marsili G, Benedetti E, Fiorentini C, Fortuna C, Pupella S, Pezzotti P, Vaglio S, Pisani G, De Angelis V, Riccardo F, Pati I. Venturi G, et al. Viruses. 2022 Mar 16;14(3):619. doi: 10.3390/v14030619. Viruses. 2022. PMID: 35337026 Free PMC article.
All samples were tested for IgM detection by the use of an ELISA test, and positive samples were tested for confirmation through the use of a PRNT. ...RESULTS: A total of 41 (0.89%) blood donors tested positive for IgM. None of these positive IgM ELISA results was c …
All samples were tested for IgM detection by the use of an ELISA test, and positive samples were tested for confirmation through the …
Infectious Burden and Semen Parameters.
Moretti E, Figura N, Campagna MS, Iacoponi F, Gonnelli S, Collodel G. Moretti E, et al. Urology. 2017 Feb;100:90-96. doi: 10.1016/j.urology.2016.10.032. Epub 2016 Oct 25. Urology. 2017. PMID: 27793655
Transmission electron microscopy data were quantified with a mathematical formula providing numerical scores, such as fertility index (FI, number of sperm free from ultrastructural defects) and the percentages of sperm apoptosis, immaturity, and necrosis. Serum samples were exami …
Transmission electron microscopy data were quantified with a mathematical formula providing numerical scores, such as fertility index (FI, n …
Possible role for IL-40 and IL-40-producing cells in the lymphocytic infiltrated salivary glands of patients with primary Sjögren's syndrome.
Guggino G, Rizzo C, Mohammadnezhad L, Lo Pizzo M, Lentini VL, Di Liberto D, La Barbera L, Raimondo S, Shekarkar Azgomi M, Urzì O, Berardicurti O, Campisi G, Alessandro R, Giacomelli R, Dieli F, Ciccia F. Guggino G, et al. RMD Open. 2023 May;9(2):e002738. doi: 10.1136/rmdopen-2022-002738. RMD Open. 2023. PMID: 37137540 Free PMC article.
MSG cellular sources of IL-40 were determined by flow-cytometry and immunofluorescence. Serum concentration of IL-40 was assessed by ELISA and cellular sources of IL-40 were determined by flow-cytometry. An in vitro assay with recombinant IL-40 (rIL-40) was performe …
MSG cellular sources of IL-40 were determined by flow-cytometry and immunofluorescence. Serum concentration of IL-40 was assessed by ELIS …
Validation of an indirect ELISA employing a chimeric recombinant gag and env peptide for the serological diagnosis of equine infectious anemia.
Scicluna MT, Autorino GL, Nogarol C, Ricci I, Frontoso R, Rosone F, Nardini R. Scicluna MT, et al. J Virol Methods. 2018 Jan;251:111-117. doi: 10.1016/j.jviromet.2017.10.002. Epub 2017 Oct 3. J Virol Methods. 2018. PMID: 28986292
The National Reference Center for equine infectious anemia (EIA) validated a commercial ELISA (Eradikit() EIAV Indirect ELISA, In3diagnostic(), Turin, Italy) employing a chimeric recombinant gag and env peptide for the detection of EIA virus antibodies, following th …
The National Reference Center for equine infectious anemia (EIA) validated a commercial ELISA (Eradikit() EIAV Indirect ELISA, …
Chronic inflammatory demyelinating polyneuropathy and HEV antibody status: A case-control study from Lazio, Italy.
Moret F, Spada E, Ceccanti M, Libonati L, D'Andrea E, Villano U, Madonna E, Chionne P, Carocci A, Pisani G, Fionda L, Antonini G, Petrucci A, Bruni R, Ciccaglione AR, Taliani G, Rivano Capparuccia M, Nobile-Orazio E, Inghilleri M, Cambieri C. Moret F, et al. J Neurol Sci. 2024 Apr 15;459:122959. doi: 10.1016/j.jns.2024.122959. Epub 2024 Mar 12. J Neurol Sci. 2024. PMID: 38490091 Free article.
MATERIALS AND METHODS: 82 CIDP and 260 from the general population have provided some personal information (demographics, anamnestic data and recognized risk factors for HEV infection) and underwent venipuncture blood sampling for virological assays testing for anti-HEV IgG and I …
MATERIALS AND METHODS: 82 CIDP and 260 from the general population have provided some personal information (demographics, anamnestic data an …
The Nuts and Bolts of SARS-CoV-2 Spike Receptor-Binding Domain Heterologous Expression.
Maffei M, Montemiglio LC, Vitagliano G, Fedele L, Sellathurai S, Bucci F, Compagnone M, Chiarini V, Exertier C, Muzi A, Roscilli G, Vallone B, Marra E. Maffei M, et al. Biomolecules. 2021 Dec 2;11(12):1812. doi: 10.3390/biom11121812. Biomolecules. 2021. PMID: 34944456 Free PMC article.
In addition, their functionality and receptor-binding ability were further evaluated through ELISA, flow cytometry assays and bio-layer interferometry....
In addition, their functionality and receptor-binding ability were further evaluated through ELISA, flow cytometry assays and bio-lay …
Evaluation of six serological ELISA kits available in Italy as screening tests for equine infectious anaemia surveillance.
Nardini R, Autorino GL, Issel CJ, Cook RF, Ricci I, Frontoso R, Rosone F, Scicluna MT. Nardini R, et al. BMC Vet Res. 2017 Apr 14;13(1):105. doi: 10.1186/s12917-017-1007-6. BMC Vet Res. 2017. PMID: 28410613 Free PMC article.
BACKGROUND: ELISAs are known to have a higher diagnostic sensitivity than the agar gel immunodiffusion (AGID) when employed for serological diagnosis of equine infectious anaemia (EIA). ...CONCLUSION: All ELISAs having high Se and precocity are preferable as a scree …
BACKGROUND: ELISAs are known to have a higher diagnostic sensitivity than the agar gel immunodiffusion (AGID) when employed for serol …
Timeline of SARS-CoV-2 Spread in Italy: Results from an Independent Serological Retesting.
Montomoli E, Apolone G, Manenti A, Boeri M, Suatoni P, Sabia F, Marchianò A, Bollati V, Pastorino U, Sozzi G. Montomoli E, et al. Viruses. 2021 Dec 30;14(1):61. doi: 10.3390/v14010061. Viruses. 2021. PMID: 35062265 Free PMC article.
Given the importance of this evidence, an independent evaluation was recommended by the World Health Organization (WHO) to test a subset of samples selected on the level of positivity in ELISA assays (positive, low positive, negative) detected in our previous study of prep …
Given the importance of this evidence, an independent evaluation was recommended by the World Health Organization (WHO) to test a subset of …
Hepatitis E Virus Occurrence in Pigs Slaughtered in Italy.
Chelli E, Suffredini E, De Santis P, De Medici D, Di Bella S, D'Amato S, Gucciardi F, Guercio A, Ostanello F, Perrone V, Purpari G, Scavia GS, Schembri P, Varcasia BM, Di Bartolo I. Chelli E, et al. Animals (Basel). 2021 Jan 22;11(2):277. doi: 10.3390/ani11020277. Animals (Basel). 2021. PMID: 33499160 Free PMC article.
Among animals collected in one abattoir, 4 out of 91 plasma tested positive for HEV-RNA. ELISA tests for the detection of total antibodies against HEV showed a high seroprevalence (76.8%), confirming the frequent exposure of pigs to the virus. ...
Among animals collected in one abattoir, 4 out of 91 plasma tested positive for HEV-RNA. ELISA tests for the detection of total antib …
52 results