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Severity of neurological Long-COVID symptoms correlates with increased level of autoantibodies targeting vasoregulatory and autonomic nervous system receptors.
Seibert FS, Stervbo U, Wiemers L, Skrzypczyk S, Hogeweg M, Bertram S, Kurek J, Anft M, Westhoff TH, Babel N. Seibert FS, et al. Autoimmun Rev. 2023 Nov;22(11):103445. doi: 10.1016/j.autrev.2023.103445. Epub 2023 Sep 7. Autoimmun Rev. 2023. PMID: 37689093 Review.
While autoantibodies specific for the vasoregulation associated Adrenergic Receptor (ADR) B1 and B2 and the CNS and vasoregulation associated muscarinic acetylcholine receptor (CHR) M3 and M4 were measured by ELISA, neurological disorders were quantified by internationally …
While autoantibodies specific for the vasoregulation associated Adrenergic Receptor (ADR) B1 and B2 and the CNS and vasoregulation associate …
Hormone Production by Human First-Trimester Gonads in a Functional In Vitro System.
Albalushi H, Sahlin L, Åkesson E, Kurek M, Kjartansdóttir KR, Lindh R, Söder O, Rotstein E, Hovatta O, Stukenborg JB. Albalushi H, et al. Endocrinology. 2019 Jan 1;160(1):133-142. doi: 10.1210/en.2018-00734. Endocrinology. 2019. PMID: 30418555
Assays for testosterone (liquid chromatography-tandem mass spectrometry), anti-Mullerian hormone (AMH; ELISA), and inhibin B (ELISA) were performed using media collected after 7 and 14 days of culture. ...
Assays for testosterone (liquid chromatography-tandem mass spectrometry), anti-Mullerian hormone (AMH; ELISA), and inhibin B (ELIS …
The reduced level of growth factors in an animal model of depression is accompanied by regulated necrosis in the frontal cortex but not in the hippocampus.
Kucharczyk M, Kurek A, Pomierny B, Detka J, Papp M, Tota K, Budziszewska B. Kucharczyk M, et al. Psychoneuroendocrinology. 2018 Aug;94:121-133. doi: 10.1016/j.psyneuen.2018.05.008. Epub 2018 May 14. Psychoneuroendocrinology. 2018. PMID: 29775875
We utilized several molecular techniques, including western blot, ELISA, quantitative RT-PCR, and various biochemical assays, to examine a range of proteins and subjected rats to behavioral tests to evaluate potential maladaptive alterations. ...
We utilized several molecular techniques, including western blot, ELISA, quantitative RT-PCR, and various biochemical assays, to exam …
Detection and diagnostic value of urine leucine-rich α-2-glycoprotein in children with suspected acute appendicitis.
Kentsis A, Ahmed S, Kurek K, Brennan E, Bradwin G, Steen H, Bachur R. Kentsis A, et al. Ann Emerg Med. 2012 Jul;60(1):78-83.e1. doi: 10.1016/j.annemergmed.2011.12.015. Epub 2012 Feb 2. Ann Emerg Med. 2012. PMID: 22305331 Free PMC article. Clinical Trial.
STUDY OBJECTIVE: Previously, we used a proteomics approach for the discovery of new diagnostic markers of acute appendicitis and identified leucine-rich alpha-2-glycoprotein (LRG) that was elevated in the urine of children with acute appendicitis and enriched in diseased appendic …
STUDY OBJECTIVE: Previously, we used a proteomics approach for the discovery of new diagnostic markers of acute appendicitis and identified …
Hypothalamic insulin and glucagon-like peptide-1 levels in an animal model of depression and their effect on corticotropin-releasing hormone promoter gene activity in a hypothalamic cell line.
Detka J, Ślusarczyk J, Kurek A, Kucharczyk M, Adamus T, Konieczny P, Kubera M, Basta-Kaim A, Lasoń W, Budziszewska B. Detka J, et al. Pharmacol Rep. 2019 Apr;71(2):338-346. doi: 10.1016/j.pharep.2018.11.001. Epub 2018 Nov 27. Pharmacol Rep. 2019. PMID: 30831439
METHODS: Adult male PS rats were subjected to acute stress and/or received orally glucose. Levels of hormones and their receptors were assayed with ELISA method. In vitro studies were performed on mHypoA-2/12 hypothalamic cell line, stably transfected with CRH promoter cou …
METHODS: Adult male PS rats were subjected to acute stress and/or received orally glucose. Levels of hormones and their receptors were assay …
Anti-lubricin monoclonal antibodies created using lubricin-knockout mice immunodetect lubricin in several species and in patients with healthy and diseased joints.
Ai M, Cui Y, Sy MS, Lee DM, Zhang LX, Larson KM, Kurek KC, Jay GD, Warman ML. Ai M, et al. PLoS One. 2015 Feb 2;10(2):e0116237. doi: 10.1371/journal.pone.0116237. eCollection 2015. PLoS One. 2015. PMID: 25642942 Free PMC article.
We determined each mAb's binding epitope, sensitivity, and specificity using biologic samples and recombinant lubricin sub-domains, and we also developed a competition ELISA assay to measure lubricin in synovial fluid and blood. We found the mAbs all recognized epit …
We determined each mAb's binding epitope, sensitivity, and specificity using biologic samples and recombinant lubricin sub-domains, and we a …
Expression, localization and systemic concentration of vascular endothelial growth factor (VEGF) and its receptors in patients with ulcerative colitis.
Frysz-Naglak D, Fryc B, Klimacka-Nawrot E, Mazurek U, Suchecka W, Kajor M, Kurek J, Stadnicki A. Frysz-Naglak D, et al. Int Immunopharmacol. 2011 Feb;11(2):220-5. doi: 10.1016/j.intimp.2010.11.023. Epub 2010 Nov 27. Int Immunopharmacol. 2011. PMID: 21115119
The localization and level of VEGF protein and its receptors protein in intestinal tissue were estimated by immunohistochemistry. VEGF concentration in serum and plasma was determined by ELISA. We found a significant increase of VEGF gene expression and increase expression …
The localization and level of VEGF protein and its receptors protein in intestinal tissue were estimated by immunohistochemistry. VEGF conce …
Inflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartum.
Brodzki P, Kostro K, Brodzki A, Wawron W, Marczuk J, Kurek Ł. Brodzki P, et al. Theriogenology. 2015 Jul 1;84(1):11-8. doi: 10.1016/j.theriogenology.2015.02.006. Epub 2015 Feb 12. Theriogenology. 2015. PMID: 25765299

The levels of the following cytokines: TNF-alpha, IL-6, IL-10 and acute-phase proteins: Hp and SAA were determined using ELISA. Our study reported that the levels of TNF-alpha, IL-6, IL-10, Hp, and SAA at 22 DPP were higher in cows with subclinical endometritis (P < 0.0

…
The levels of the following cytokines: TNF-alpha, IL-6, IL-10 and acute-phase proteins: Hp and SAA were determined using ELISA. Our s …
Antibodies against toxic shock syndrome toxin no. 1 (TSST-1) in Poland.
Bulanda M, Kunstmann G, Mauff G, Kurek M, Pulverer G, Heczko PB. Bulanda M, et al. Zentralbl Bakteriol Mikrobiol Hyg A. 1989 Jan;270(3):396-9. doi: 10.1016/s0176-6724(89)80008-2. Zentralbl Bakteriol Mikrobiol Hyg A. 1989. PMID: 2929191
A total of 174 sera from healthy Polish individuals of different age groups were tested for TSST-1 antibody by the micro-ELISA method. Over 90% of sera contained the antibody with titres equal to above 1:10(2), even in children and adolescents. ...
A total of 174 sera from healthy Polish individuals of different age groups were tested for TSST-1 antibody by the micro-ELISA method …