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Year Number of Results
1998 1
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2009 3
2010 1
2011 2
2012 1
2013 1
2014 3
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34 results

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Page 1
How we diagnose the antiphospholipid syndrome.
Giannakopoulos B, Passam F, Ioannou Y, Krilis SA. Giannakopoulos B, et al. Blood. 2009 Jan 29;113(5):985-94. doi: 10.1182/blood-2007-12-129627. Epub 2008 Aug 28. Blood. 2009. PMID: 18755986 Free article. Review.
Particular focus is given to anti-beta 2-glycoprotein I (beta(2)GPI) antibodies in view of their recent inclusion in the APS classification criteria. The clinical utility of using the beta(2)GPI enzyme-linked immunosorbent assay, in conjunction with th …
Particular focus is given to anti-beta 2-glycoprotein I (beta(2)GPI) antibodies in view of their recent inclusion in the APS classification …
Premature T cell aging in major depression: A double hit by the state of disease and cytomegalovirus infection.
Simon MS, Ioannou M, Arteaga-Henríquez G, Wijkhuijs A, Berghmans R, Musil R, Müller N, Drexhage HA. Simon MS, et al. Brain Behav Immun Health. 2023 Feb 27;29:100608. doi: 10.1016/j.bbih.2023.100608. eCollection 2023 May. Brain Behav Immun Health. 2023. PMID: 36909830 Free PMC article.
In a second FACS analysis, naive, effector memory (Tem), central memory (Tcm), effector memory cells re-expressing RA (TEMRA), as well as CD28(+) and CD27(+) memory populations, were determined of the CD4(+) and CD8(+) T cell populations in a subsample (N = 35 MDD and N = 36 HC). …
In a second FACS analysis, naive, effector memory (Tem), central memory (Tcm), effector memory cells re-expressing RA (TEMRA), as well as CD …
Development of an ELISA for the quantification of the C-terminal decapeptide prothymosin alpha(100-109) in sera of mice infected with bacteria.
Samara P, Kalbacher H, Ioannou K, Radu DL, Livaniou E, Promponas VJ, Voelter W, Tsitsilonis O. Samara P, et al. J Immunol Methods. 2013 Sep 30;395(1-2):54-62. doi: 10.1016/j.jim.2013.06.011. Epub 2013 Jul 4. J Immunol Methods. 2013. PMID: 23831611
Thus, using high-affinity-purified polyclonal antibodies (Abs), raised in rabbits and a prototype antibody-capture system, we developed a highly sensitive and specific competitive ELISA for proTalpha(100-109). The sensitivity of the ELISA (0.1ng/mL to 10mug/mL) is a …
Thus, using high-affinity-purified polyclonal antibodies (Abs), raised in rabbits and a prototype antibody-capture system, we developed a hi …
Altered DNA methylation pattern characterizes the peripheral immune cells of patients with autoimmune hepatitis.
Zachou K, Arvaniti P, Lyberopoulou A, Sevdali E, Speletas M, Ioannou M, Koukoulis GK, Renaudineau Y, Dalekos GN. Zachou K, et al. Liver Int. 2022 Jun;42(6):1355-1368. doi: 10.1111/liv.15176. Epub 2022 Feb 17. Liver Int. 2022. PMID: 35108441
Global DNA methylation (5(m) C)/hydroxymethylation (5(hm) C) was studied by ELISAs. mRNA of DNA methylation (DNMT1/3A/3B) and their counteracting hydroxymethylation enzymes (TET1/2/3) was determined by quantitative RT-PCR. ...
Global DNA methylation (5(m) C)/hydroxymethylation (5(hm) C) was studied by ELISAs. mRNA of DNA methylation (DNMT1/3A/3B) and their c …
Laboratory tests for the antiphospholipid syndrome.
Pericleous C, Ripoll VM, Giles I, Ioannou Y. Pericleous C, et al. Methods Mol Biol. 2014;1134:221-35. doi: 10.1007/978-1-4939-0326-9_17. Methods Mol Biol. 2014. PMID: 24497366
The clinical diagnosis of APS in a patient with symptoms and signs is dependent upon the presence of a persistently positive result in an aPL assay. The tests recommended for detecting aPL are the standardized enzyme-linked immunosorbent assay ( …
The clinical diagnosis of APS in a patient with symptoms and signs is dependent upon the presence of a persistently positive result in an aP …
Development of a sensitive cost-effective capture ELISA for detection of murine monoclonal antibodies: correlation with SPR biosensor technology.
Tsiamita M, Pavlaki M, Toloudi M, Tiptiri-Kourpeti A, Drossou V, Apostolou P, Chatziioannou M, Ioannou E, Papasotiriou I, Chlichlia K. Tsiamita M, et al. Antiinflamm Antiallergy Agents Med Chem. 2012;11(2):173-81. doi: 10.2174/187152312803305722. Antiinflamm Antiallergy Agents Med Chem. 2012. PMID: 22882081
Production of monoclonal antibodies requires the development of fast, high-throughput methodologies for screening and selecting appropriate candidate antibodies for development. Capture (sandwich) enzyme linked immunosorbent assay (ELISA) provid …
Production of monoclonal antibodies requires the development of fast, high-throughput methodologies for screening and selecting appropriate …
Peripheral alpha-synuclein levels in patients with genetic and non-genetic forms of Parkinson's disease.
Emmanouilidou E, Papagiannakis N, Kouloulia S, Galaziou A, Antonellou R, Papadimitriou D, Athanasiadou A, Bozi M, Koros C, Maniati M, Vekrellis K, Ioannou PC, Stefanis L. Emmanouilidou E, et al. Parkinsonism Relat Disord. 2020 Apr;73:35-40. doi: 10.1016/j.parkreldis.2020.03.014. Epub 2020 Mar 22. Parkinsonism Relat Disord. 2020. PMID: 32234683
Levels of total alpha-synuclein were assessed using an in-house ELISA assay. RESULTS: A statistically significant increase of alpha-synuclein levels was found in serum, but not plasma, from GU-PD patients compared to healthy controls. ...
Levels of total alpha-synuclein were assessed using an in-house ELISA assay. RESULTS: A statistically significant increase of …
Assessment of α-synuclein secretion in mouse and human brain parenchyma.
Emmanouilidou E, Elenis D, Papasilekas T, Stranjalis G, Gerozissis K, Ioannou PC, Vekrellis K. Emmanouilidou E, et al. PLoS One. 2011;6(7):e22225. doi: 10.1371/journal.pone.0022225. Epub 2011 Jul 14. PLoS One. 2011. PMID: 21779395 Free PMC article.
However, proof that alpha-synuclein is actually secreted into the brain extracellular space in vivo has not been obtained. We developed a novel highly sensitive ELISA in conjugation with an in vivo microdialysis technique to measure alpha-synuclein in brain interstitial fl …
However, proof that alpha-synuclein is actually secreted into the brain extracellular space in vivo has not been obtained. We developed a no …
Beta 2 glycoprotein I is a substrate of thiol oxidoreductases.
Passam FH, Rahgozar S, Qi M, Raftery MJ, Wong JW, Tanaka K, Ioannou Y, Zhang JY, Gemmell R, Qi JC, Giannakopoulos B, Hughes WE, Hogg PJ, Krilis SA. Passam FH, et al. Blood. 2010 Sep 16;116(11):1995-7. doi: 10.1182/blood-2010-02-271494. Blood. 2010. PMID: 20847215 Free article. No abstract available.
34 results