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An inverted repeat transgene with a structure that cannot generate double-stranded RNA, suffers silencing independent of DNA methylation.
Skårn M, Eike MC, Meza TJ, Mercy IS, Jakobsen KS, Aalen RB. Skårn M, et al. Transgenic Res. 2006 Aug;15(4):489-500. doi: 10.1007/s11248-006-0019-y. Transgenic Res. 2006. PMID: 16906449
GC content, putative matrix-attachment regions and transcriptional interference from neighbouring genes could all be ruled out as major causes of silencing. ...Also, the methylation pattern deviated from that characteristic for RNA-mediated DNA methylation and silen …
GC content, putative matrix-attachment regions and transcriptional interference from neighbouring genes could all be ruled out as maj …