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Digital PCR can augment the interpretation of RT-qPCR Cq values for SARS-CoV-2 diagnostics.
Whale AS, von der Heide EK, Kohlenberg M, Brinckmann A, Baedker S, Karalay O, Fernandez-Gonzalez A, Busby EJ, Bustin SA, Hauser H, Missel A, O'Sullivan DM, Huggett JF, Pfaffl MW, Nolan T. Whale AS, et al. Among authors: o sullivan dm. Methods. 2022 May;201:5-14. doi: 10.1016/j.ymeth.2021.08.006. Epub 2021 Aug 26. Methods. 2022. PMID: 34454016 Free PMC article.
An inter-laboratory study to investigate the impact of the bioinformatics component on microbiome analysis using mock communities.
O'Sullivan DM, Doyle RM, Temisak S, Redshaw N, Whale AS, Logan G, Huang J, Fischer N, Amos GCA, Preston MD, Marchesi JR, Wagner J, Parkhill J, Motro Y, Denise H, Finn RD, Harris KA, Kay GL, O'Grady J, Ransom-Jones E, Wu H, Laing E, Studholme DJ, Benavente ED, Phelan J, Clark TG, Moran-Gilad J, Huggett JF. O'Sullivan DM, et al. Sci Rep. 2021 May 19;11(1):10590. doi: 10.1038/s41598-021-89881-2. Sci Rep. 2021. PMID: 34012005 Free PMC article.