Green biorefinery: Microalgae-bacteria microbiome on tolerance investigations in plants

Dong Bui-Xuan; Doris Ying Ying Tang; Kit Wayne Chew; Thi Dong Phuong Nguyen; Han Le Ho; Thi Ngoc Thu Tran; Toan Nguyen-Sy; Thi Ha Thuong Dinh; Phuc Son Nguyen; Thi My Huong Dinh; Thanh Thuy Nguyen; Pau Loke Show

doi:10.1016/j.jbiotec.2021.12.002

Green biorefinery: Microalgae-bacteria microbiome on tolerance investigations in plants

J Biotechnol. 2022 Jan 10:343:120-127. doi: 10.1016/j.jbiotec.2021.12.002. Epub 2021 Dec 8.

Authors

Affiliations

¹ The University of Danang, University of Science and Technology, 54 Nguyen Luong Bang St., Danang 550 000, Viet Nam.
² Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
³ School of Energy and Chemical Engineering, Xiamen University Malaysia, Jalan Sunsuria, Bandar Sunsuria, 43900 Sepang, Selangor Darul Ehsan, Malaysia; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China. Electronic address: kitwayne.chew@xmu.edu.my.
⁴ The University of Danang, University of Technology and Education, 48 Cao Thang St., Danang 550 000, Viet Nam. Electronic address: ntdphuong@ute.udn.vn.
⁵ Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeonbuk 56212, Republic of Korea; University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
⁶ The University of Danang, University of Technology and Education, 48 Cao Thang St., Danang 550 000, Viet Nam.
⁷ The University of Danang, University of Science and Education, 459 Ton Duc Thang St., Danang 550 000, Viet Nam.
⁸ National Institute of Hygiene and Epidemiology, 01 Yecxanh St, Hanoi, Viet Nam.

PMID: 34896159
DOI: 10.1016/j.jbiotec.2021.12.002

Abstract

Co-culture of microalgae and microorganisms, supported with the resulting synergistic effects, can be used for wastewater treatment, biomass production, agricultural applications and etc. Therefore, this study aimed to explore the role of Bacillus subtilis (B. subtilis) in tolerance against the harsh environment of seafood wastewater, at which these microalgal-bacterial flocs were formed by microalgae cultivation. In this present study, B. subtilis isolated from the cultivation medium of Chlorella vulgaris and exposed to different salinity (0.1-4% w/v sodium chloride) and various pH range to determine the tolerant ability and biofilm formation. Interestingly, this bacteria strain that isolated from microalgae cultivation medium showed the intense viability in the salt concentration exceeding up to 4% (w/v) NaCl but demonstrated the decrease in cell division as environmental culture undergoing over pH 10. Cell viability was recorded higher than 71% and 92% for B. subtilis inoculum in media with salt concentration greater than 20 gL^-1 and external pH 6.5-9, respectively. This showed that B. subtilis isolated from microalgal-bacteria cocultivation exhibited its tolerant ability to survive in the extremely harsh conditions and thus, mitigating the stresses due to salinity and pH.

Keywords: Bacillus sp.; Biofilm; Microalgae; PH tolerance; Salinity tolerance.

Publication types

Review

MeSH terms

Bacillus subtilis
Biomass
Chlorella vulgaris*
Microalgae*
Microbiota*
Wastewater

Substances

Waste Water