Early, Real-Time Medical Diagnosis of Botulism by Endopeptidase-Mass Spectrometry

Osnat Rosen; Liron Feldberg; Sigalit Gura; Tal Brosh-Nissimov; Alex Guri; Oren Zimhony; Eli Shapiro; Adi Beth-Din; Dana Stein; Eyal Ozeri; Ada Barnea; Amram Turgeman; Alon Ben David; Arieh Schwartz; Eytan Elhanany; Eran Diamant; Shmuel Yitzhaki; Ran Zichel

doi:10.1093/cid/civ861

Early, Real-Time Medical Diagnosis of Botulism by Endopeptidase-Mass Spectrometry

Clin Infect Dis. 2015 Dec 15;61(12):e58-61. doi: 10.1093/cid/civ861. Epub 2015 Sep 28.

Authors

Osnat Rosen¹, Liron Feldberg², Sigalit Gura², Tal Brosh-Nissimov³, Alex Guri⁴, Oren Zimhony⁴, Eli Shapiro⁵, Adi Beth-Din⁶, Dana Stein⁶, Eyal Ozeri¹, Ada Barnea¹, Amram Turgeman¹, Alon Ben David¹, Arieh Schwartz¹, Eytan Elhanany⁶, Eran Diamant¹, Shmuel Yitzhaki⁷, Ran Zichel¹

Affiliations

¹ Department of Biotechnology.
² Department of Analytical Chemistry.
³ CBRN Medicine Branch, Medical Corps HQ, Israel Institute for Biological Research, Ness Ziona.
⁴ Infectious Diseases Unit.
⁵ Pediatric Intensive Care Unit, Kaplan Medical Center, Rehovot.
⁶ Department of Biochemistry and Molecular Genetics.
⁷ Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel.

PMID: 26420800
DOI: 10.1093/cid/civ861

Abstract

Botulinum toxin was detected in patient serum using Endopeptidase-mass-spectrometry assay, although all conventional tests provided negative results. Antitoxin was administered, resulting in patient improvement. Implementing this highly sensitive and rapid assay will improve preparedness for foodborne botulism and deliberate exposure.

Keywords: Endopep-MS; botulism; diagnosis.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Antitoxins / administration & dosage
Botulism / diagnosis*
Botulism / therapy
Early Diagnosis
Endopeptidases / blood*
Humans
Infant
Male
Mass Spectrometry / methods*
Serum / chemistry
Time Factors
Treatment Outcome

Substances

Antitoxins
Endopeptidases