Objective: To determine the susceptibility genes and resistance genes in HLA-DRB1 alleles in Tibetan patients with cystic and alveolar hydatid diseases, so as to provide the references for the research of the genetic characteristics and infection mechanism of Tibetan hydatid diseases.
Methods: The case control method was applied. The Tibetan patients with cystic and alveolar hydatid diseases (63 and 73 cases respectively) in Yushu and Guoluo Tibetan Autonomous Prefecture, and unrelated healthy people (60 cases) in this area were selected as the study subjects. The polymerase chain reaction-sequence based typing (PCR-SBT) technique was applied for genotyping of HLA-DRB1, and the comparison of the gene frequency.
Results: The frequency of HLA-DRB1*04 in the alveolar/cystic echinococcosis group was lower than that in the control group ( χ2 = 4.71, 4.31, both P < 0.05).
Conclusions: HLA-DRB1*04 genotypes may be associated with the resistance of cystic and alveolar echinococcosis and its resistance genes.
[摘要]目的 确定青藏高原地区藏族人群包虫病患者的人类白细胞抗原-DRB1 (Human leukocyte antigen-DRB1, HLA-DRB1) 等位基因中易感基因和抗性基因, 为研究藏族人群包虫病的感染机制及遗传特点提供依据。 方法 本研究采用 病例对照法。病例组选取青海省玉树和果洛藏族自治州的世居藏族囊型包虫病63例和泡型包虫病73例; 对照组选取该 地区无血缘关系的藏族健康人, 共计60例。应用聚合酶链反应-直接碱基序列基因分型 (PCR-SBT) 技术, 比较等位基因 频率。 结果 泡型和囊型包虫病病例组HLA-DRB1*04等位基因频率均小于对照组 (χ2 = 4.71、4.31, P均< 0.05)。 结论 HLA-DRB1*04等位基因与青藏高原藏族人群囊型和泡型包虫病相关联, 对其感染有保护作用, 是其抗性基因。.
Keywords: Association study; Echinococcosis; HLA-DRB1; Resistance gene; Susceptibility; Tibetan Plateau.