Characterization and antioxidant properties of six Algerian propolis extracts: ethyl acetate extracts inhibit myeloperoxidase activity

Int J Mol Sci. 2014 Feb 7;15(2):2327-45. doi: 10.3390/ijms15022327.

Abstract

Because propolis contains many types of antioxidant compounds such as polyphenols and flavonoids, it can be useful in preventing oxidative damages. Ethyl acetate extracts of propolis from several Algerian regions show high activity by scavenging free radicals, preventing lipid peroxidation and inhibiting myeloperoxidase (MPO). By fractioning and assaying ethyl acetate extracts, it was observed that both polyphenols and flavonoids contribute to these activities. A correlation was observed between the polyphenol content and the MPO inhibition. However, it seems that kaempferol, a flavonoid, contributes mainly to the MPO inhibition. This molecule is in a high amount in the ethyl acetate extract and demonstrates the best efficiency towards the enzyme with an inhibiting concentration at 50% of 4 ± 2 µM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry*
  • Antioxidants / pharmacology*
  • Ascorbic Acid / chemistry
  • Enzyme Activation / drug effects
  • Flavonoids / chemistry
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology
  • Inhibitory Concentration 50
  • Lipid Peroxidation / drug effects
  • Liquid-Liquid Extraction
  • Oxidation-Reduction / drug effects
  • Peroxidase / antagonists & inhibitors
  • Polyphenols / chemistry
  • Propolis / chemistry*

Substances

  • Antioxidants
  • Flavonoids
  • Free Radical Scavengers
  • Polyphenols
  • Propolis
  • Peroxidase
  • Ascorbic Acid