Multiresponse optimization of a UPLC method for the simultaneous determination of tryptophan and 15 tryptophan-derived compounds using a Box-Behnken design with a desirability function

Widiastuti Setyaningsih; Irfan E Saputro; Ceferino A Carrera; Miguel Palma; Carmelo G Barroso

doi:10.1016/j.foodchem.2016.12.034

Multiresponse optimization of a UPLC method for the simultaneous determination of tryptophan and 15 tryptophan-derived compounds using a Box-Behnken design with a desirability function

Food Chem. 2017 Jun 15:225:1-9. doi: 10.1016/j.foodchem.2016.12.034. Epub 2016 Dec 16.

Authors

Widiastuti Setyaningsih¹, Irfan E Saputro², Ceferino A Carrera², Miguel Palma³, Carmelo G Barroso²

Affiliations

¹ Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Gadjah Mada University, Jalan Flora, Bulaksumur, 55281 Yogyakarta, Indonesia; Department of Analytical Chemistry, Faculty of Sciences, Instituto de Investigación Vitivinícola y Agroalimentaria (IVAGRO), University of Cadiz, Campus de Excelencia Internacional Agroalimentario (CeiA3), Campus del Rio San Pedro, 11510, Puerto Real, Cádiz, Spain.
² Department of Analytical Chemistry, Faculty of Sciences, Instituto de Investigación Vitivinícola y Agroalimentaria (IVAGRO), University of Cadiz, Campus de Excelencia Internacional Agroalimentario (CeiA3), Campus del Rio San Pedro, 11510, Puerto Real, Cádiz, Spain.
³ Department of Analytical Chemistry, Faculty of Sciences, Instituto de Investigación Vitivinícola y Agroalimentaria (IVAGRO), University of Cadiz, Campus de Excelencia Internacional Agroalimentario (CeiA3), Campus del Rio San Pedro, 11510, Puerto Real, Cádiz, Spain. Electronic address: miguel.palma@uca.es.

PMID: 28193401
DOI: 10.1016/j.foodchem.2016.12.034

Abstract

A Box-Behnken design was used in conjunction with multiresponse optimization based on the desirability function to carry out the simultaneous separation of tryptophan and 15 derivatives by Ultra Performance Liquid Chromatography. The gradient composition of the mobile phase and the flow rate were optimized with respect to the resolution of severely overlapping chromatographic peaks and the total run time. Two different stationary phases were evaluated (hybrid silica and a solid-core-based C₁₈ column). The methods were validated and a suitable sensitivity was found for all compounds in the concentration range 1-100μgL^-1 (R²>0.999). High levels of repeatability and intermediate precision (CV less than 0.25% and 1.7% on average for the retention time and the signal area, respectively) were obtained. The new method was applied to the determination tryptophan and its derivatives in black pigmented glutinous and non-glutinous rice grain samples.

Keywords: 1-Methyl-l-tryptophan (PubChem CID: 676159); 3-Indolepropionic acid (PubChem CID: 3744); 5-Hydroxy-l-tryptophan (PubChem CID: 439280); 5-Methoxy-dl-tryptophan (PubChem CID: 119802); 5-Methoxytryptamine (PubChem CID: 6198); Chromatographic separation; Design of experiments; Indole-3-acetonitrile (PubChem CID: 351795); Indole-3-butyric acid (PubChem CID: 273800); Indole-3-carboxylic acid (PubChem CID: 69867); Kynurenine (PubChem CID: 846); Melatonin (PubChem CID: 896); N-Acetyl-5-hydroxytryptamine (PubChem CID: 903); N-acetyl-l-tryptophan (PubChem CID: 700653); Serotonin (PubChem CID: 5202); Tryptamine (PubChem CID: 1150); Tryptophan; Tryptophan derivatives; l-Tryptophan (PubChem CID: 6305).

MeSH terms

Chromatography, High Pressure Liquid / methods*
Tryptophan / analogs & derivatives*
Tryptophan / analysis

Substances

Tryptophan