Optimization of a Binary Concrete Crack Self-Healing System Containing Bacteria and Oxygen

Jinlong Zhang; Bixia Mai; Tingwei Cai; Jiayi Luo; Wanhan Wu; Bing Liu; Ningxu Han; Feng Xing; Xu Deng

doi:10.3390/ma10020116

Optimization of a Binary Concrete Crack Self-Healing System Containing Bacteria and Oxygen

Materials (Basel). 2017 Jan 26;10(2):116. doi: 10.3390/ma10020116.

Authors

Jinlong Zhang^{1

2

3}, Bixia Mai⁴, Tingwei Cai⁵, Jiayi Luo⁶, Wanhan Wu⁷, Bing Liu⁸, Ningxu Han⁹, Feng Xing^{2

3}, Xu Deng¹⁰

Affiliations

¹ Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. xingf@szu.edu.cn.
² Guangdong Province Key Laboratory of Durability for Marine Civil Engineering, The Key Laboratory on Durability of Civil Engineering in Shenzhen, School of Civil Engineering, Shenzhen University, Shenzhen 518060, China. xingf@szu.edu.cn.
³ Key Laboratory of Earthquake Engineering and Engineering Vibration, Institute of Engineering Mechanics, China Earthquake Administration, Harbin 150086, China. xingf@szu.edu.cn.
⁴ Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. 871830180max@gmail.com.
⁵ Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. 2015300071@email.szu.edu.cn.
⁶ Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. 2015300183@email.szu.edu.cn.
⁷ Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. 201430004@email.szu.edu.cn.
⁸ Guangdong Province Key Laboratory of Durability for Marine Civil Engineering, The Key Laboratory on Durability of Civil Engineering in Shenzhen, School of Civil Engineering, Shenzhen University, Shenzhen 518060, China. liubing0708@szu.edu.cn.
⁹ Guangdong Province Key Laboratory of Durability for Marine Civil Engineering, The Key Laboratory on Durability of Civil Engineering in Shenzhen, School of Civil Engineering, Shenzhen University, Shenzhen 518060, China. nxhan@szu.edu.cn.
¹⁰ Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. dengxu@szu.edu.cn.

Abstract

An optimized strategy for the enhancement of microbially induced calcium precipitation including spore viability ensurance, nutrient selection and O2 supply was developed. Firstly, an optimal yeast extract concentration of 5 g/l in sporulation medium was determined based on viable spore yield and spore viability. Furthermore, the effects of certain influential factors on microbial calcium precipitation process of H4 in the presence of oxygen releasing tablet (ORT) were evaluated. The results showed that CaO2 is preferable to other peroxides in improving the calcium precipitation by H4. H4 strain is able to precipitate a highly insoluble calcium at the CaO2 dosage range of 7.5-12.5 g/l, and the most suitable spore concentration is 6 × 108 spores/ml when the spore viability (viable spore ratio) is approximately 50%. Lactate is the best carbon source and nitrate is the best nitrogen source for aerobic incubation. This work has laid a foundation of ternary self-healing system containing bacteria, ORT, and nutrients, which will be promising for the self-healing of cracks deep inside the concrete structure.

Keywords: Bacillus sp.; concrete crack; microbially induced calcium precipitation; oxygen supply; self healing; spore vialility.