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Subcutaneous immunization with inactivated bacterial components and purified protein of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevents puerperal metritis in Holstein dairy cows.
Machado VS, Bicalho ML, Meira Junior EB, Rossi R, Ribeiro BL, Lima S, Santos T, Kussler A, Foditsch C, Ganda EK, Oikonomou G, Cheong SH, Gilbert RO, Bicalho RC. Machado VS, et al. PLoS One. 2014 Mar 17;9(3):e91734. doi: 10.1371/journal.pone.0091734. eCollection 2014. PLoS One. 2014. PMID: 24638139 Free PMC article.
Inactivated whole cells were produced using two genetically distinct strains of each bacterial species (E. coli, F. necrophorum, and T. pyogenes). FimH and PLO subunits were produced using recombinant protein expression, and LKT was recovered from culturing a wild F
Inactivated whole cells were produced using two genetically distinct strains of each bacterial species (E. coli, F. necrophorum, and …