Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation

Biomolecules. 2020 Feb 11;10(2):276. doi: 10.3390/biom10020276.

Abstract

The lack of oxygen and post-anoxic reactions cause significant alterations of plant growth and metabolism. Plant hormones are active participants in these alterations. This study focuses on auxin-a phytohormone with a wide spectrum of effects on plant growth and stress tolerance. The indoleacetic acid (IAA) content in plants was measured by ELISA. The obtained data revealed anoxia-induced accumulation of IAA in wheat and rice seedlings related to their tolerance of oxygen deprivation. The highest IAA accumulation was detected in rice roots. Subsequent reoxygenation was accompanied with a fast auxin reduction to the control level. A major difference was reported for shoots: wheat seedlings contained less than one-third of normoxic level of auxin during post-anoxia, while IAA level in rice seedlings rapidly recovered to normoxic level. It is likely that the mechanisms of auxin dynamics resulted from oxygen-induced shift in auxin degradation and transport. Exogenous IAA treatment enhanced plant survival under anoxia by decreased electrolyte leakage, production of hydrogen peroxide and lipid peroxidation. The positive effect of external IAA application coincided with improvement of tolerance to oxygen deprivation in the 35S:iaaM × 35S:iaaH lines of transgene tobacco due to its IAA overproduction.

Keywords: auxin; oxygen deficiency; post-anoxia; rice; tobacco; wheat.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrolytes / analysis
  • Electrolytes / metabolism
  • Hydrogen Peroxide / metabolism
  • Indoleacetic Acids / analysis*
  • Indoleacetic Acids / metabolism
  • Indoleacetic Acids / pharmacology*
  • Lipid Peroxidation / drug effects
  • Nicotiana / genetics
  • Nicotiana / metabolism
  • Oryza / chemistry*
  • Oryza / drug effects
  • Oryza / metabolism
  • Oxidative Stress
  • Oxygen / pharmacology*
  • Plant Growth Regulators / analysis
  • Plant Growth Regulators / metabolism
  • Plant Growth Regulators / pharmacology
  • Plant Roots / chemistry
  • Plant Roots / metabolism
  • Plant Shoots / chemistry
  • Plant Shoots / metabolism
  • Plants, Genetically Modified
  • Seedlings / chemistry*
  • Seedlings / drug effects
  • Seedlings / metabolism
  • Triticum / chemistry*
  • Triticum / drug effects
  • Triticum / metabolism

Substances

  • Electrolytes
  • Indoleacetic Acids
  • Plant Growth Regulators
  • Hydrogen Peroxide
  • Oxygen