Spatial Organization of Gene Expression in Systems of Cellular Differentiation and Autoimmune Diseases

The importance of the spatial distribution of genes and gene regulation areas in an organism's genome has seen an increased interest in studies in the fields of genetics and epigenetics.This work attempts to apply an already established pipeline for topological and functional analysis of gene expression on a system of cell differentiation of mice embryonic cells exposed to TCF3.The analysis includes a separation of genes in different categories based on their expression's behavior over time, the functional analysis in overexpressed and underexpressed genes, the identification of domains of focal regulation/deregulation, and a network analysis between genes and functional categories in different time points.The results show two major transition points regarding the up- and downregulation in gene expression. Considering the enrichment pathways, apart from those related to development and morphogenesis, a couple more chromosomal regions show behaviors worthy of further examination.