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Acetone production in solventogenic Clostridium species: new insights from non-enzymatic decarboxylation of acetoacetate.
Han B, Gopalan V, Ezeji TC. Han B, et al. Appl Microbiol Biotechnol. 2011 Aug;91(3):565-76. doi: 10.1007/s00253-011-3276-5. Epub 2011 Apr 28. Appl Microbiol Biotechnol. 2011. PMID: 21533580
Our fermentation studies revealed that this mutant produces a maximum acetone concentration of 3 g/L (in P2 medium), a value comparable to that produced by wild-type C. beijerinckii 8052. Therefore, we postulated that AADC-catalyzed decarboxylation of acetoacetate is not t …
Our fermentation studies revealed that this mutant produces a maximum acetone concentration of 3 g/L (in P2 medium), a value comparable to t …
Allopurinol-mediated lignocellulose-derived microbial inhibitor tolerance by Clostridium beijerinckii during acetone-butanol-ethanol (ABE) fermentation.
Ujor V, Agu CV, Gopalan V, Ezeji TC. Ujor V, et al. Appl Microbiol Biotechnol. 2015 Apr;99(8):3729-40. doi: 10.1007/s00253-015-6450-3. Epub 2015 Feb 18. Appl Microbiol Biotechnol. 2015. PMID: 25690312
Since NAD(P)H/NAD(P)(+) levels in C. beijerinckii were largely unchanged upon allopurinol treatment, we postulated and validated a possible basis in DNA repair to account for the solventogenic gains with allopurinol. ...Consistent with this finding, addition of inosine (a …
Since NAD(P)H/NAD(P)(+) levels in C. beijerinckii were largely unchanged upon allopurinol treatment, we postulated and validated a po …
Identification, purification and characterization of furfural transforming enzymes from Clostridium beijerinckii NCIMB 8052.
Zhang Y, Ujor V, Wick M, Ezeji TC. Zhang Y, et al. Anaerobe. 2015 Jun;33:124-31. doi: 10.1016/j.anaerobe.2015.03.005. Epub 2015 Mar 18. Anaerobe. 2015. PMID: 25796546
Although Clostridium beijerinckii NCIMB 8052 can transform 2-3 g/L of furfural and HMF to their less toxic alcohols, higher concentrations present in biomass hydrolysates are intractable to microbial transformation. To delineate the mechanism by which C. beijerinckii detox …
Although Clostridium beijerinckii NCIMB 8052 can transform 2-3 g/L of furfural and HMF to their less toxic alcohols, higher concentrations p …
Use of proteomic analysis to elucidate the role of calcium in acetone-butanol-ethanol fermentation by Clostridium beijerinckii NCIMB 8052.
Han B, Ujor V, Lai LB, Gopalan V, Ezeji TC. Han B, et al. Appl Environ Microbiol. 2013 Jan;79(1):282-93. doi: 10.1128/AEM.02969-12. Epub 2012 Oct 26. Appl Environ Microbiol. 2013. PMID: 23104411 Free PMC article.
Toward an understanding of the basis for these pleiotropic effects, we profiled changes in the C. beijerinckii NCIMB 8052 proteome that occur in response to the addition of CaCO(3). ...
Toward an understanding of the basis for these pleiotropic effects, we profiled changes in the C. beijerinckii NCIMB 8052 proteome th …
Butanol production from agricultural residues: Impact of degradation products on Clostridium beijerinckii growth and butanol fermentation.
Ezeji T, Qureshi N, Blaschek HP. Ezeji T, et al. Biotechnol Bioeng. 2007 Aug 15;97(6):1460-9. doi: 10.1002/bit.21373. Biotechnol Bioeng. 2007. PMID: 17274071
In these studies we investigated the effect of some of the lignocellulosic hydrolysate inhibitors associated with C. beijerinckii BA101 growth and acetone-butanol-ethanol (ABE) production. When 0.3 g/L rho-coumaric and ferulic acids were introduced into the fermentation me …
In these studies we investigated the effect of some of the lignocellulosic hydrolysate inhibitors associated with C. beijerinckii BA1 …
Elucidating and alleviating impacts of lignocellulose-derived microbial inhibitors on Clostridium beijerinckii during fermentation of Miscanthus giganteus to butanol.
Zhang Y, Ezeji TC. Zhang Y, et al. J Ind Microbiol Biotechnol. 2014 Oct;41(10):1505-16. doi: 10.1007/s10295-014-1493-5. Epub 2014 Aug 2. J Ind Microbiol Biotechnol. 2014. PMID: 25085743
Fermentation of liquid hot water (LHW) pretreated Miscanthus giganteus (MG) by Clostridium beijerinckii NCIMB 8052 was investigated towards understanding the toxicity of lignocellulose-derived inhibitors to solventogenic Clostridium species vis-a-vis butanol production. While …
Fermentation of liquid hot water (LHW) pretreated Miscanthus giganteus (MG) by Clostridium beijerinckii NCIMB 8052 was investigated towards …
Continuous butanol fermentation and feed starch retrogradation: butanol fermentation sustainability using Clostridium beijerinckii BA101.
Ezeji TC, Qureshi N, Blaschek HP. Ezeji TC, et al. J Biotechnol. 2005 Jan 26;115(2):179-87. doi: 10.1016/j.jbiotec.2004.08.010. J Biotechnol. 2005. PMID: 15607236
The continuous reactor fed with cornstarch solution (feed temperature 19 degrees C) produced approximately 6.0 gL(-1) total ABE. Increasing the feed storage temperature to 37 degrees C improved ABE production to 7.2 gL(-1) suggesting that retrogradation was occurrin …
The continuous reactor fed with cornstarch solution (feed temperature 19 degrees C) produced approximately 6.0 gL(-1) total ABE. Incr …
Biotransformation of furfural and 5-hydroxymethyl furfural (HMF) by Clostridium acetobutylicum ATCC 824 during butanol fermentation.
Zhang Y, Han B, Ezeji TC. Zhang Y, et al. N Biotechnol. 2012 Feb 15;29(3):345-51. doi: 10.1016/j.nbt.2011.09.001. Epub 2011 Sep 10. N Biotechnol. 2012. PMID: 21925629
The effect of furfural and HMF on butanol production by Clostridium acetobutylicum 824 was investigated. Whereas specific growth rates, mu, of C. acetobutylicum in the presence of furfural and HMF were in the range of 15-85% and 23-78%, respectively, of the uninhibited Con …
The effect of furfural and HMF on butanol production by Clostridium acetobutylicum 824 was investigated. Whereas specific growth rates, mu, …
Glycerol supplementation of the growth medium enhances in situ detoxification of furfural by Clostridium beijerinckii during butanol fermentation.
Ujor V, Agu CV, Gopalan V, Ezeji TC. Ujor V, et al. Appl Microbiol Biotechnol. 2014;98(14):6511-21. doi: 10.1007/s00253-014-5802-8. Epub 2014 May 17. Appl Microbiol Biotechnol. 2014. PMID: 24839212
For example, the fermentation medium supplemented with glycerol and subjected to 5 g/L furfural elicited up to 1.8- and 3-fold increases, respectively, in NADH and NADPH levels in C. beijerinckii 8052 relative to the control culture. These critical changes are the likely u …
For example, the fermentation medium supplemented with glycerol and subjected to 5 g/L furfural elicited up to 1.8- and 3-fold increases, re …
Bioproduction of butanol in bioreactors: new insights from simultaneous in situ butanol recovery to eliminate product toxicity.
Mariano AP, Qureshi N, Filho RM, Ezeji TC. Mariano AP, et al. Biotechnol Bioeng. 2011 Aug;108(8):1757-65. doi: 10.1002/bit.23123. Epub 2011 Mar 15. Biotechnol Bioeng. 2011. PMID: 21370232
Integrated batch fermentations and ABE recovery were conducted at 37 C using a 14-L bioreactor (7.0 L fermentation volume) containing initial substrate (glucose) concentration of 60 g/L. ...A control ABE fermentation experiment was characterized by incomplete glucose utili …
Integrated batch fermentations and ABE recovery were conducted at 37 C using a 14-L bioreactor (7.0 L fermentation volume) containing …
14 results