Chiral separation of natural and unnatural amino acid derivatives by micro-HPLC on a Ristocetin A stationary phase

Anna-Maria Piccinini; Martin G Schmid; Tamara Pajpanova; Svetlana Pancheva; Ekatarina Grueva; Gerald Gübitz

doi:10.1016/j.jbbm.2004.02.004

Chiral separation of natural and unnatural amino acid derivatives by micro-HPLC on a Ristocetin A stationary phase

J Biochem Biophys Methods. 2004 Oct 29;61(1-2):11-21. doi: 10.1016/j.jbbm.2004.02.004.

Authors

Anna-Maria Piccinini¹, Martin G Schmid, Tamara Pajpanova, Svetlana Pancheva, Ekatarina Grueva, Gerald Gübitz

Affiliation

¹ Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, Karl-Franzens University, Universitätsplatz 1, A-8010 Graz, Austria.

PMID: 15560918
DOI: 10.1016/j.jbbm.2004.02.004

Abstract

This paper deals with the chiral separation of Fmoc- and Z-derivatives of natural and unnatural sulfur containing amino acids by micro-HPLC. The separations were carried out in microbore columns packed with a new material based on Ristocetin A bonded to 3.5 microm silica gel. The columns were run in the normal phase, polar organic mode as well as in the reversed phase mode, whereby best results were obtained with the reversed-phase mode using mixtures of triethylamine acetate (TEAA) buffer and methanol as mobile phases.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / chemistry
Amino Acids / isolation & purification
Amino Acids, Sulfur / chemistry*
Amino Acids, Sulfur / isolation & purification*
Chromatography, High Pressure Liquid / methods*
Fluorenes / chemistry
Fluorenes / isolation & purification
Isomerism
Microfluidics / instrumentation
Microfluidics / methods*
Ristocetin / chemistry*

Substances

Amino Acids
Amino Acids, Sulfur
Fluorenes
N(alpha)-fluorenylmethyloxycarbonylamino acids
Ristocetin
ristocetin A