Quantitative Analysis and Monitoring of EZH2 Mutations Using Liquid Biopsy in Follicular Lymphoma

Genes (Basel). 2020 Jul 13;11(7):785. doi: 10.3390/genes11070785.

Abstract

Recent advances in molecular technologies enable sensitive and quantitative assessment of circulating tumor DNA, offering a noninvasive disease monitoring tool for patients with malignant disorders. Here, we demonstrated on four follicular lymphoma cases that circulating tumor DNA based EZH2 mutation analysis performed by a highly sensitive droplet digital PCR method may be a valuable treatment monitoring approach in EZH2 mutant follicular lymphoma. EZH2 variant allele frequencies changed in parallel with the volume of metabolically active tumor sites observed on 18F-fluorodeoxyglucose positron emission tomography combined with computer tomography (PET-CT) scans. Variant allele frequencies of EZH2 mutations decreased or were eliminated rapidly upon successful treatment, with treatment failure being associated with elevated EZH2 variant allele frequencies. We also demonstrated spatial heterogeneity in a patient with two different EZH2 mutations in distinct anatomical sites, with both mutations simultaneously detected in the liquid biopsy specimen. In summary, circulating tumor DNA based EZH2 mutation analysis offers a rapid, real-time, radiation-free monitoring tool for sensitive detection of EZH2 mutations deriving from different anatomical sites in follicular lymphoma patients receiving immunochemotherapy.

Keywords: EZH2; circulating tumor DNA; droplet digital PCR; follicular lymphoma; liquid biopsy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Biomarkers, Tumor / genetics*
  • Circulating Tumor DNA / genetics*
  • DNA Mutational Analysis
  • Enhancer of Zeste Homolog 2 Protein / genetics*
  • Female
  • Fluorodeoxyglucose F18 / chemistry
  • Humans
  • Liquid Biopsy
  • Lymphoma, Follicular / blood
  • Lymphoma, Follicular / genetics*
  • Lymphoma, Follicular / pathology
  • Male
  • Middle Aged
  • Mutation / genetics

Substances

  • Biomarkers, Tumor
  • Circulating Tumor DNA
  • Fluorodeoxyglucose F18
  • EZH2 protein, human
  • Enhancer of Zeste Homolog 2 Protein