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Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme.
Kubiak X, Polsinelli I, Chavas LMG, Fyfe CD, Guillot A, Fradale L, Brewee C, Grimaldi S, Gerbaud G, Thureau A, Legrand P, Berteau O, Benjdia A. Kubiak X, et al. Nat Chem Biol. 2024 Mar;20(3):382-391. doi: 10.1038/s41589-023-01493-1. Epub 2023 Dec 29. Nat Chem Biol. 2024. PMID: 38158457
Here we report an atomic-resolution crystal structure of a RiPP-modifying radical SAM enzyme in complex with its substrate properly positioned in the active site. ...Collectively, our study brings unique perspectives on how radical SAM enzymes interact with RiPPs an …
Here we report an atomic-resolution crystal structure of a RiPP-modifying radical SAM enzyme in complex with its substrate properly p …
Crystal structure and functional mapping of human ASMT, the last enzyme of the melatonin synthesis pathway.
Botros HG, Legrand P, Pagan C, Bondet V, Weber P, Ben-Abdallah M, Lemière N, Huguet G, Bellalou J, Maronde E, Beguin P, Haouz A, Shepard W, Bourgeron T. Botros HG, et al. J Pineal Res. 2013 Jan;54(1):46-57. doi: 10.1111/j.1600-079X.2012.01020.x. Epub 2012 Jul 9. J Pineal Res. 2013. PMID: 22775292
The polypeptide chain of ASMT consists of a C-terminal domain, which is typical of other SAM-dependent O-methyltransferases, and an N-terminal domain, which intertwines several helices with another monomer to form the physiologically active dimer. ...
The polypeptide chain of ASMT consists of a C-terminal domain, which is typical of other SAM-dependent O-methyltransferases, and an N …
On sulfur core level binding energies in thiol self-assembly and alternative adsorption sites: An experimental and theoretical study.
Jia J, Kara A, Pasquali L, Bendounan A, Sirotti F, Esaulov VA. Jia J, et al. J Chem Phys. 2015 Sep 14;143(10):104702. doi: 10.1063/1.4929350. J Chem Phys. 2015. PMID: 26374051
Calculated differences in S(2p) CLBEs for free BDMT molecules, SH group sulfur on top of the SAM, and disulfide are also reported to clarify possible errors in assignments....
Calculated differences in S(2p) CLBEs for free BDMT molecules, SH group sulfur on top of the SAM, and disulfide are also reported to …