M gene targeted qRT-PCR approach for SARS-CoV-2 virus detection.
Sarkar MMH, Naser SR, Chowdhury SF, Khan MS, Habib MA, Akter S, Banu TA, Goswami B, Jahan I, Nayem MR, Hassan MA, Khan MI, Rabbi MFA, Ahsan CR, Miah MI, Nessa A, Islam SMRU, Rahman MA, Shaikh MAA, Ahmed MS.
Sarkar MMH, et al.
Sci Rep. 2023 Oct 3;13(1):16659. doi: 10.1038/s41598-023-43204-9.
Sci Rep. 2023.
PMID: 37789078
Free PMC article.
The limit of detection was determined using successive dilutions of known copies of SARS-CoV-2 pseudovirus. The M gene based assay was able to detect a minimum of 100 copies of virus/mL indicating its capacity to detect low viral load. The assay showed comparable accuracy, …
The limit of detection was determined using successive dilutions of known copies of SARS-CoV-2 pseudovirus. The M gene based assay wa …