Bioimaging of a chromenoquinoline-based ratiometric fluorescent probe for detecting ClO

Huimin Shangguan; Qianwei Liu; Yuanjuan Wang; Zixuan Teng; Rumeng Tian; Tong Wu; Lei Yang; Ling Jiang; Xingjiang Liu; Liuhe Wei

doi:10.1016/j.saa.2023.123256

Bioimaging of a chromenoquinoline-based ratiometric fluorescent probe for detecting ClO

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15:303:123256. doi: 10.1016/j.saa.2023.123256. Epub 2023 Aug 11.

Authors

Huimin Shangguan¹, Qianwei Liu², Yuanjuan Wang³, Zixuan Teng⁴, Rumeng Tian⁴, Tong Wu³, Lei Yang⁵, Ling Jiang⁶, Xingjiang Liu⁷, Liuhe Wei⁴

Affiliations

¹ College of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
² International College, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
³ Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Medicine, Linyi University, Linyi 276000, China.
⁴ Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
⁵ Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Medicine, Linyi University, Linyi 276000, China. Electronic address: yanglei@lyu.edu.cn.
⁶ Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Medicine, Linyi University, Linyi 276000, China. Electronic address: jiangling@lyu.edu.cn.
⁷ Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan Province, China. Electronic address: xingjiangliu@zzu.edu.cn.

PMID: 37579661
DOI: 10.1016/j.saa.2023.123256

Abstract

Hypochlorous acid (HClO) is a reactive oxygen species and a relatively strong antibacterial substance in the immune defense system. The normal concentration of HClO in the human body is approximately 200 μM, and its high concentration can cause tissue damage and some diseases. Herein, a chromenoquinoline-based ratiometric fluorescent probe was developed to detect and quantify HClO. The developed Probe 1 exhibited the advantages of large Stokes shift (137 nm), high synthetic yield (84.7 %), simple synthesis method, short response time (<4 min), low detection limit (5.1 nM), and low toxicity. The probe was successfully validated in live cells and zebrafish.

Keywords: Bioimaging; Fluorescent probe; Hypochlorous acid; Ratiometric.

MeSH terms

Animals
Fluorescent Dyes*
Humans
Hypochlorous Acid
Zebrafish*

Substances

Fluorescent Dyes
Hypochlorous Acid