Comparing High-Frequency With Monophasic Electroporation Protocols in an In Vivo Beating Heart Model

Eyal Heller; Tomas Garcia-Sanchez; Yonatan Moshkovits; Raul Rabinovici; Dvora Grynberg; Amit Segev; Samuel J Asirvatham; Antoni Ivorra; Elad Maor

doi:10.1016/j.jacep.2021.05.003

Comparing High-Frequency With Monophasic Electroporation Protocols in an In Vivo Beating Heart Model

JACC Clin Electrophysiol. 2021 Aug;7(8):959-964. doi: 10.1016/j.jacep.2021.05.003. Epub 2021 Jun 30.

Authors

Eyal Heller¹, Tomas Garcia-Sanchez², Yonatan Moshkovits¹, Raul Rabinovici³, Dvora Grynberg¹, Amit Segev⁴, Samuel J Asirvatham⁵, Antoni Ivorra⁶, Elad Maor⁷

Affiliations

¹ Neufeld and Tamman Cardiovascular Research Institutes, Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel; Heart Center, Sheba Medical Center, Tel Hashomer, Israel.
² Department of Information and Communication Technologies, Universitat Pompeu Fabra, Barcelona, Spain.
³ Department of Electrical and Computer Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
⁴ Neufeld and Tamman Cardiovascular Research Institutes, Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel; Heart Center, Sheba Medical Center, Tel Hashomer, Israel; Department of Electrical and Computer Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
⁵ Department of Cardiovascular Medicine, Mayo Clinic, Rochester, Minnesota, USA.
⁶ Department of Information and Communication Technologies, Universitat Pompeu Fabra, Barcelona, Spain; Serra Húnter Fellow Programme, Universitat Pompeu Fabra, Barcelona, Spain.
⁷ Neufeld and Tamman Cardiovascular Research Institutes, Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel; Heart Center, Sheba Medical Center, Tel Hashomer, Israel. Electronic address: Elad.Maor@Sheba.health.gov.il.

PMID: 34217666
DOI: 10.1016/j.jacep.2021.05.003

Abstract

This study compared monophasic 100-μs pulses with high-frequency electroporation (HF-EP) bursts using an in vivo animal model. Myocardial damage was evaluated by histologic analysis. Compared with 10 monophasic pulses, 20 bursts of HF-EP at 100 and 150 kHz were associated with less damage. However, when the number of HF-EP bursts was increased to 60, myocardial damage was comparable to that of the monophasic group. HF-EP protocols were associated with attenuated collateral muscle contractions. This study shows that HF-EP is feasible and effective and that pulse frequency has a significant effect on extent of ablation.

Keywords: H-FIRE; electroporation; muscle contraction; pulsed field ablation.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Electroporation*
Heart*
Myocardium