A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity

R Vikneswaran; Muhamad Syamir Syafiq; Naser Eltaher Eltayeb; Mohd Naqiuddin Kamaruddin; S Ramesh; R Yahya

doi:10.1016/j.saa.2015.05.087

A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity

Spectrochim Acta A Mol Biomol Spectrosc. 2015:150:175-80. doi: 10.1016/j.saa.2015.05.087. Epub 2015 May 30.

Authors

R Vikneswaran¹, Muhamad Syamir Syafiq², Naser Eltaher Eltayeb³, Mohd Naqiuddin Kamaruddin², S Ramesh⁴, R Yahya⁵

Affiliations

¹ Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. Electronic address: r.vikneswaran@yahoo.com.my.
² Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia.
³ Department of Chemistry, Sciences & Arts College - Rabigh, King Abdulaziz University, Rabigh, Saudi Arabia.
⁴ Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. Electronic address: rameshtsubra@gmail.com.
⁵ Department of Chemistry, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia. Electronic address: rosiyah@um.edu.my.

PMID: 26046495
DOI: 10.1016/j.saa.2015.05.087

Abstract

Copper ion recognition and DNA interaction of a newly synthesized fluorescent Schiff base (HPyETSC) were investigated using UV-vis and fluorescent spectroscopy. Examination using these two techniques revealed that the detection of copper by HPyETSC is highly sensitive and selective, with a detection limit of 0.39 μm and the mode of interaction between HPyETSC and DNA is electrostatic, with a binding constant of 8.97×10(4) M(-1). Furthermore, gel electrophoresis studies showed that HPyETSC exhibited nuclease activity through oxidative pathway.

Keywords: Chemical nucleases; Chemosensor; DNA binding; Fluorescent; Thiosemicarbazone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Copper / analysis*
Copper / isolation & purification
DNA / drug effects
DNA / metabolism*
DNA Cleavage / drug effects
Deoxyribonucleases / chemical synthesis
Deoxyribonucleases / chemistry
Deoxyribonucleases / pharmacology*
Fluorescent Dyes* / chemical synthesis
Fluorescent Dyes* / chemistry
Fluorescent Dyes* / pharmacology
Limit of Detection
Schiff Bases / chemical synthesis
Schiff Bases / chemistry*
Schiff Bases / pharmacology*
Spectrometry, Fluorescence
Sulfhydryl Compounds / chemistry
Sulfhydryl Compounds / pharmacology
Thiourea / analogs & derivatives
Thiourea / chemical synthesis
Thiourea / chemistry
Thiourea / pharmacology

Substances

Fluorescent Dyes
Schiff Bases
Sulfhydryl Compounds
Copper
DNA
Deoxyribonucleases
Thiourea