Genome-Wide Analysis of the Biosynthesis and Deactivation of Gibberellin-Dioxygenases Gene Family in Camellia sinensis (L.) O. Kuntze

Cheng Pan; Kunhong Tian; Qiuyan Ban; Leigang Wang; Qilu Sun; Yan He; Yuanfei Yang; Yuting Pan; Yeyun Li; Jiayue Jiang; Changjun Jiang

doi:10.3390/genes8090235

Genome-Wide Analysis of the Biosynthesis and Deactivation of Gibberellin-Dioxygenases Gene Family in Camellia sinensis (L.) O. Kuntze

Genes (Basel). 2017 Sep 19;8(9):235. doi: 10.3390/genes8090235.

Authors

Cheng Pan¹, Kunhong Tian², Qiuyan Ban³, Leigang Wang⁴, Qilu Sun⁵, Yan He⁶, Yuanfei Yang⁷, Yuting Pan⁸, Yeyun Li⁹, Jiayue Jiang¹⁰, Changjun Jiang^{11

12}

Affiliations

¹ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. panchengtt@163.com.
² State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. tiankh@ahau.edu.cn.
³ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. banqiuyan717@163.com.
⁴ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. 15720340@ahau.edu.cn.
⁵ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. sunql@ahau.edu.cn.
⁶ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. heyan@ahau.edu.cn.
⁷ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. yangyf@ahau.edu.cn.
⁸ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. panyt@ahau.edu.cn.
⁹ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. lyy@ahau.edu.cn.
¹⁰ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. jiangjy@ahau.edu.cn.
¹¹ State Key Laboratory of Tea Plant Biology and Utilization, College of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China. jiangcj@ahau.edu.cn.
¹² Henan Provincial Key Laboratory of Tea Plant Biology, College of Life Sciences, Xinyang Normal University, Xinyang 464000, China. jiangcj@ahau.edu.cn.

Abstract

Gibberellins (GAs), a class of diterpenoid phytohormones, play a key role in regulating diverse processes throughout the life cycle of plants. Bioactive GA levels are rapidly regulated by Gibberellin-dioxygenases (GAox), which are involved in the biosynthesis and deactivation of gibberellin. In this manuscript, a comprehensive genome-wide analysis was carried out to find all GAox in Camellia sinensis. For the first time in a tea plant, 14 CsGAox genes, containing two domains, DIOX_N (PF14226) and 2OG-FeII_Oxy, were identified (PF03171). These genes all belong to 2-oxoglutarate-dependent dioxygenases (2-ODD), including four CsGA20ox (EC: 1.14.11.12), three CsGA3ox (EC: 1.14.11.15), and seven CsGA2ox (EC: 1.14.11.13). According to the phylogenetic classification as in Arabidopsis, the CsGAox genes spanned five subgroups. Each CsGAox shows tissue-specific expression patterns, although these vary greatly. Some candidate genes, which may play an important role in response to external abiotic stresses, have been identified with regards to patterns, such as CsGA20ox2, CsGA3ox2, CsGA3ox3, CsGA2ox1, CsGA2ox2, and CsGA2ox4. The bioactive GA levels may be closely related to the GA20ox, GA3ox and GA2ox genes. In addition, the candidate genes could be used as marker genes for abiotic stress resistance breeding in tea plants.

Keywords: Camellia sinensis; Gibberellins-dioxygenases; abiotic stress; genome-wide analysis; gibberellin.