The expression of four aflatoxin (AF) biosynthetic pathway genes (aflD, aflO, aflP and aflQ) was evaluated in 24 Aspergillus flavus strains isolated from soils of pistachio orchards, with the aim of rapidly and accurately differentiating toxigenic from non-toxigenic strains. The amounts of AFB1 produced by 20 aflatoxigenic strains varied from 1.25 to 321.56 ng/mg fungal dry weights in YES medium. RT-PCR results showed that transcription of the four genes was not always correlated with AF production. The expression pattern of aflO and aflQ, however, was found to be well correlated with the amounts of AFB1 produced when strains were arbitrarily classified into two types: type I, comprised of strains producing ≥30 ng/mg; and type II, low (≤30 ng/mg) and non-AF producers. The present study suggests that, under specific growth conditions, the expression pattern of aflatoxin biosynthetic pathway genes such as aflO and aflQ can be used to infer the AF-producing capability of A. flavus strains.
Copyright © 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.