Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates

Biosensors (Basel). 2015 Oct 28;5(4):664-77. doi: 10.3390/bios5040664.

Abstract

In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein-antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration.

Keywords: Raman spectroscopy; biomarker; protein sensing; surface-enhanced Raman scattering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aniline Compounds / chemistry
  • Antibodies, Immobilized / chemistry
  • Biomarkers / analysis
  • ErbB Receptors / analysis*
  • Humans
  • Immunoassay / methods
  • Mercaptopurine / chemistry
  • Microscopy / methods
  • Nanostructures / chemistry
  • Silver / chemistry
  • Spectrum Analysis, Raman / methods*
  • Sulfhydryl Compounds / chemistry
  • Surface Properties
  • Tumor Suppressor Protein p53 / analysis*

Substances

  • Aniline Compounds
  • Antibodies, Immobilized
  • Biomarkers
  • Sulfhydryl Compounds
  • Tumor Suppressor Protein p53
  • Silver
  • 4-aminothiophenol
  • Mercaptopurine
  • ErbB Receptors