Hypertension is a complex disorder involving multiple organ systems and the primary modifiable risk factor for heart disease. Aim of the study was to assess the relationship of serum HDL-C and triglyceride with hypertensive patients in order to compare this parameter with normotensive subjects. This analytical type of cross sectional study was carried out in the Department of Physiology, Mymensingh Medical College, Mymensingh, Bangladesh from July 2017 to June 2018. A total number of 120 male subjects, age ranged from 30-65 years was included in this study. Among them, sixty (60) hypertensive subjects were taken as study group (Group II) and sixty (60) ages matched normotensive male subjects were taken as control group (Group I). Data were expressed as mean±SD and statistical significance of difference among the group was calculated by unpaired student's 't' test. In this study we found that serum triglyceride and serum HDL cholesterol were significant in study group in comparison with control group. Therefore, by this study we recommended that routine estimation of these parameters is important for prevention of complication related to hypertension for leading a healthy life.