Differences on salivary proteome at rest and in response to an acute exercise in men and women: A pilot study

Lorena Franco-Martínez; Jorge Miguel González-Hernández; Anita Horvatić; Nicolas Guillemin; José Joaquín Cerón; Silvia Martínez-Subiela; Miguel Ángel Sentandreu; Mirna Brkljačić; Vladimir Mrljak; Asta Tvarijonaviciute; Pedro Jiménez Reyes

doi:10.1016/j.jprot.2019.103629

Differences on salivary proteome at rest and in response to an acute exercise in men and women: A pilot study

J Proteomics. 2020 Mar 1:214:103629. doi: 10.1016/j.jprot.2019.103629. Epub 2019 Dec 24.

Affiliations

¹ Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia 30100, Spain. Electronic address: lorena.franco2@um.es.
² Faculty of Health Sciences, Universidad Europea de Canarias, Tenerife, Spain.
³ ERA Chair FP7, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
⁴ Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia 30100, Spain. Electronic address: jjceron@um.es.
⁵ Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia 30100, Spain. Electronic address: silviams@um.es.
⁶ Instituto de Agroquímica y Tecnología de Alimentos (CSIC), 46980 Paterna, Valencia, Spain. Electronic address: ciesen@iata.csic.es.
⁷ ERA Chair FP7, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia. Electronic address: mbrkljacic@vef.hr.
⁸ ERA Chair FP7, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia. Electronic address: vmrljak@vef.hr.
⁹ Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia 30100, Spain. Electronic address: asta@um.es.
¹⁰ Centre for Sport Studies, King Juan Carlos University, Madrid, Spain.

PMID: 31881350
DOI: 10.1016/j.jprot.2019.103629

Abstract

The aim of the present study was to evaluate the differences in salivary proteome at rest and in response to an acute exercise in men and women. For this, unstimulated whole saliva samples in rest and after a bout resistance exercise leading to failure (ELF) of both men and women (n = 5 for each sex) were subjected to isobaric Tandem Mass Tags (TMT) labelling followed by LC-MS/MS. A total of 274 proteins were identified and met the inclusion criteria. 16 proteins were modulated for the interaction sex*exercise, 6 were modulated because of the exercise, and 65 were differentially expressed between men and women at rest. In conclusion, these results indicate sex-related differences in the salivary proteome at rest and after an acute exercise, pointing out possible candidate biomarkers for sports performance and allowing further knowledge of the physiological processes occurring during ELF. SIGNIFICANCE: The present study describes for the first time the changes that occur in salivary proteome detected by TMT-based proteomics in response to an acute exercise and the differences in these changes depending on sex. Of the 274 protein identified, the 87 differentially expressed proteins and their related pathways were discussed, focusing on the sex- and exercise-related differences in the salivary proteome.

Keywords: Exercise; Label-based proteomics; Resistance training; Saliva; Sex influence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid
Exercise
Female
Humans
Male
Pilot Projects
Proteome*
Saliva
Salivary Proteins and Peptides
Tandem Mass Spectrometry*

Substances

Proteome
Salivary Proteins and Peptides