RORγT is overexpressed in iNKT and γδ T cells during relapse in relapsing-remitting multiple sclerosis

J Neuroimmunol. 2019 Dec 15:337:577046. doi: 10.1016/j.jneuroim.2019.577046. Epub 2019 Aug 28.

Abstract

The aim of the current study is to evaluate IL-17 production and RORγT, and IL-23R expression by iNKT, Th17 and γδ T cells in the peripheral blood of relapsing-remitting multiple sclerosis patients. Samples of peripheral blood from 21 relapse patients and 12 remission patients, and 15 healthy volunteers were stained with monoclonal antibodies for flow cytometry analysis. No significant differences in iNKT, γδ T and Th17 percentages were noted. The significant overexpression of RORγT was observed in all three subpopulations - therefore, iNKT, γδ T and Th cells may be an important source of IL-17 shortly prior to the relapse.

Keywords: IL-17; Multiple sclerosis; RORγT; Th17; iNKT; γδ T.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Female
  • Gene Expression
  • Humans
  • Intraepithelial Lymphocytes / immunology
  • Intraepithelial Lymphocytes / metabolism*
  • Male
  • Middle Aged
  • Multiple Sclerosis, Relapsing-Remitting / genetics
  • Multiple Sclerosis, Relapsing-Remitting / immunology
  • Multiple Sclerosis, Relapsing-Remitting / metabolism*
  • Natural Killer T-Cells / immunology
  • Natural Killer T-Cells / metabolism*
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / biosynthesis*
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / genetics
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / immunology

Substances

  • Nuclear Receptor Subfamily 1, Group F, Member 3
  • RORC protein, human