DDAO Controlled Synthesis of Organo-Modified Silica Nanoparticles with Encapsulated Fluorescent Boron Dipyrrins and Study of Their Uptake by Cancerous Cells

Molecules. 2020 Aug 21;25(17):3802. doi: 10.3390/molecules25173802.

Abstract

The design of cargo carriers with high biocompatibility, unique morphological characteristics, and capability of strong bonding of fluorescent dye is highly important for the development of a platform for smart imaging and diagnostics. In this paper, BODIPY-doped silica nanoparticles were prepared through a "one-pot" soft-template method using a sol-gel process. Several sol-gel precursors have been used in sol-gel synthesis in the presence of soft-template to obtain the silica-based materials with the most appropriate morphological features for the immobilization of BODIPY molecules. Obtained silica particles have been shown to be non-cytotoxic and can be effectively internalized into the cervical cancer cell line (HeLa). The described method of synthesis allows us to obtain silica-based carriers with an immobilized fluorescent dye that provide the possibility for real-time imaging and detection of these carriers.

Keywords: BODIPY; oil-in-water; one-pot synthesis; silica nanoparticles; soft-template.

MeSH terms

  • Boron / chemistry*
  • Boron Compounds / chemistry*
  • Cell Survival
  • Dimethylamines / chemistry*
  • Female
  • HeLa Cells
  • Humans
  • Nanoparticles / administration & dosage*
  • Nanoparticles / chemistry
  • Phase Transition
  • Silicon Dioxide / chemistry*
  • Uterine Cervical Neoplasms / drug therapy*
  • Uterine Cervical Neoplasms / metabolism

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Dimethylamines
  • dodecyldimethylamine oxide
  • Silicon Dioxide
  • Boron