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Chitinous material bioconversion by three new chitinases from the yeast Mestchnikowia pulcherrima.
Minguet-Lobato M, Cervantes FV, Míguez N, Plou FJ, Fernández-Lobato M. Minguet-Lobato M, et al. Microb Cell Fact. 2024 Jan 20;23(1):31. doi: 10.1186/s12934-024-02300-9. Microb Cell Fact. 2024. PMID: 38245740 Free PMC article. Review.
The secretion of a chitinase activity from the ubiquitous yeast Mestchnikowia pulcherrima and their involvement in the post-harvest biological control of fungal pathogens was previously reported. RESULTS: Three new chitinases from M. pulcherrima, MpChit35, MpChit38 and MpC …
The secretion of a chitinase activity from the ubiquitous yeast Mestchnikowia pulcherrima and their involvement in the post-harvest biologic …
Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts.
Garcia-Gonzalez M, Plou FJ, Cervantes FV, Remacha M, Poveda A, Jiménez-Barbero J, Fernandez-Lobato M. Garcia-Gonzalez M, et al. Microb Biotechnol. 2019 Nov;12(6):1274-1285. doi: 10.1111/1751-7915.13490. Epub 2019 Oct 1. Microb Biotechnol. 2019. PMID: 31576667 Free PMC article.
New oligosaccharides were produced by transglucosylation processes employing M. reukaufii cell extracts in overload-sucrose reactions. These products were purified and structurally characterized by MS-ESI and NMR techniques. ...
New oligosaccharides were produced by transglucosylation processes employing M. reukaufii cell extracts in overload-sucrose reactions …
Molecular characterization and heterologous expression of two α-glucosidases from Metschnikowia spp, both producers of honey sugars.
Garcia-Gonzalez M, Minguet-Lobato M, Plou FJ, Fernandez-Lobato M. Garcia-Gonzalez M, et al. Microb Cell Fact. 2020 Jul 11;19(1):140. doi: 10.1186/s12934-020-01397-y. Microb Cell Fact. 2020. PMID: 32652991 Free PMC article.
RESULTS: In this study, genes codifying for alpha-glucosidases from the nectaries yeast M. gruessii and M. reukaufii were characterised and heterologously expressed in Escherichia coli for the first time. ...Recombinant enzymes produced oligosaccharides naturally pr …
RESULTS: In this study, genes codifying for alpha-glucosidases from the nectaries yeast M. gruessii and M. reukaufii were char …
Molecular and biochemical characterization of a beta-fructofuranosidase from Xanthophyllomyces dendrorhous.
Linde D, Macias I, Fernández-Arrojo L, Plou FJ, Jiménez A, Fernández-Lobato M. Linde D, et al. Appl Environ Microbiol. 2009 Feb;75(4):1065-73. doi: 10.1128/AEM.02061-08. Epub 2008 Dec 16. Appl Environ Microbiol. 2009. PMID: 19088319 Free PMC article.
The enzyme was able to hydrolyze fructosyl-beta-(2-->1)-linked carbohydrates such as sucrose, 1-kestose, or nystose, although its catalytic efficiency, defined by the k(cat)/K(m) ratio, indicates that it hydrolyzes sucrose approximately 4.2 times more efficiently than 1 …
The enzyme was able to hydrolyze fructosyl-beta-(2-->1)-linked carbohydrates such as sucrose, 1-kestose, or nystose, although its catalyt …
Characterization of a beta-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose.
Alvaro-Benito M, de Abreu M, Fernández-Arrojo L, Plou FJ, Jiménez-Barbero J, Ballesteros A, Polaina J, Fernández-Lobato M. Alvaro-Benito M, et al. J Biotechnol. 2007 Oct 15;132(1):75-81. doi: 10.1016/j.jbiotec.2007.07.939. Epub 2007 Jul 26. J Biotechnol. 2007. PMID: 17904238
The enzyme shows broad substrate specificity, hydrolyzing sucrose, 1-kestose, nystose and raffinose, with different catalytic efficiencies (k(cat)/K(m)). Although the main reaction catalysed by this enzyme is sucrose hydrolysis, it also produces two fructooligosaccharides …
The enzyme shows broad substrate specificity, hydrolyzing sucrose, 1-kestose, nystose and raffinose, with different catalytic efficiencies ( …
Molecular structure of the SWA2 gene encoding an AMY1-related alpha-amylase from Schwanniomyces occidentalis.
Claros MG, Abarca D, Fernández-Lobato M, Jiménez A. Claros MG, et al. Curr Genet. 1993 Jul-Aug;24(1-2):75-83. doi: 10.1007/BF00324668. Curr Genet. 1993. PMID: 8358835
It contains an open reading frame of 1521 bp which has the potential to encode a 507 amino-acid protein of M(r) 55966. Its deduced amino-acid sequence shows significant similarities to the sequence of other studied alpha-amylases. ...
It contains an open reading frame of 1521 bp which has the potential to encode a 507 amino-acid protein of M(r) 55966. Its deduced am …
Purification and biochemical characterization of an alpha-glucosidase from Xanthophyllomyces dendrorhous.
Marín D, Linde D, Fernández Lobato M. Marín D, et al. Yeast. 2006 Jan 30;23(2):117-25. doi: 10.1002/yea.1345. Yeast. 2006. PMID: 16491468
The rate parameters measured for the hydrolysis of starch and various chain length malto-oligosaccharides shows high catalytic efficiency, calculated by the relationship V(cat)/K(m), for malto-oligosaccharides, such as maltotriose (873 mM(-1) min(-1)), or maltoheptose (698 …
The rate parameters measured for the hydrolysis of starch and various chain length malto-oligosaccharides shows high catalytic efficiency, c …
New insights into the fructosyltransferase activity of Schwanniomyces occidentalis ß-fructofuranosidase, emerging from nonconventional codon usage and directed mutation.
Alvaro-Benito M, de Abreu M, Portillo F, Sanz-Aparicio J, Fernández-Lobato M. Alvaro-Benito M, et al. Appl Environ Microbiol. 2010 Nov;76(22):7491-9. doi: 10.1128/AEM.01614-10. Epub 2010 Sep 17. Appl Environ Microbiol. 2010. PMID: 20851958 Free PMC article.
Substitution of leucine for Ser196 dramatically lowers the apparent catalytic efficiency (k(cat)/K(m)) of the enzyme (approximately 1,000-fold), but surprisingly, its transferase activity is enhanced by almost 3-fold, as is the enzymes' specificity for 6-kestose synthesis. …
Substitution of leucine for Ser196 dramatically lowers the apparent catalytic efficiency (k(cat)/K(m)) of the enzyme (approximately 1 …
Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity.
Kidibule PE, Santos-Moriano P, Jiménez-Ortega E, Ramírez-Escudero M, Limón MC, Remacha M, Plou FJ, Sanz-Aparicio J, Fernández-Lobato M. Kidibule PE, et al. Microb Cell Fact. 2018 Mar 22;17(1):47. doi: 10.1186/s12934-018-0895-x. Microb Cell Fact. 2018. PMID: 29566690 Free PMC article.
Chit42 hydrolysed colloidal chitin at least 10-times more efficiently (defined by the k(cat)/K(m) ratio) than any of the assayed chitosan. Production of partially acetylated chitooligosaccharides was confirmed in reaction mixtures using HPAEC-PAD chromatography and mass sp …
Chit42 hydrolysed colloidal chitin at least 10-times more efficiently (defined by the k(cat)/K(m) ratio) than any of the assayed chit …