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A single mutation in framework 2 of the heavy variable domain improves the properties of a diabody and a related single-chain antibody.
Rodríguez-Rodríguez ER, Ledezma-Candanoza LM, Contreras-Ferrat LG, Olamendi-Portugal T, Possani LD, Becerril B, Riaño-Umbarila L. Rodríguez-Rodríguez ER, et al. Among authors: ledezma candanoza lm. J Mol Biol. 2012 Oct 26;423(3):337-50. doi: 10.1016/j.jmb.2012.07.007. Epub 2012 Jul 23. J Mol Biol. 2012. PMID: 22835504
Optimal Neutralization of Centruroides noxius Venom Is Understood through a Structural Complex between Two Antibody Fragments and the Cn2 Toxin.
Riaño-Umbarila L, Ledezma-Candanoza LM, Serrano-Posada H, Fernández-Taboada G, Olamendi-Portugal T, Rojas-Trejo S, Gómez-Ramírez IV, Rudiño-Piñera E, Possani LD, Becerril B. Riaño-Umbarila L, et al. Among authors: ledezma candanoza lm. J Biol Chem. 2016 Jan 22;291(4):1619-1630. doi: 10.1074/jbc.M115.685297. Epub 2015 Nov 20. J Biol Chem. 2016. PMID: 26589800 Free PMC article.