Magnetic graphene nanocomposites were prepared by hydrothermal synthesis, and aniline polymerization was initiated by magnetic graphene. These polyaniline/magnetic graphene (PANI/MG) composites were used to immobilize laccase to construct biosensors. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and infrared spectroscopy (IR) were used to characterize these composites. Cyclic voltammetry and chronoamperometry technique were used to test the electrical properties of the constructed polyaniline/magnetic graphene laccase modified electrode. The results show that the polyaniline/magnetic graphene immobilizing laccase modified electrode exhibited superior electrical properties, including high sensitivity, detection limit and linear range. The hydroquinone was used as an analytical and detection probe. The selectivity was 0.03639 A/(mol/L), the linear range was 0.4-337.2 μmol/L, and the detection limit was 2.94 μM (signal/noise = 3, minimum identification value of effective signal). The biosensor can reach the conditions for detecting the actual water sample.
Keywords: Graphene; Laccase; Sensor.
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