HPLC Analysis and Cytotoxicity of n-Butanol Extract from Glyphaea brevis Roots Against C6 Glioma Cells

Koffi Marcel Konan; Janat Akhanovna Mamyrbekova-Bekro; Norbert Bakalara; David Virieux; Jean-Luc Pirat; Yves-Alain Bekro

doi:10.3797/scipharm.1307-08

HPLC Analysis and Cytotoxicity of n-Butanol Extract from Glyphaea brevis Roots Against C6 Glioma Cells

Sci Pharm. 2013 Nov 19;82(1):171-6. doi: 10.3797/scipharm.1307-08. Print 2014 Jan-Mar.

Authors

Koffi Marcel Konan¹, Janat Akhanovna Mamyrbekova-Bekro¹, Norbert Bakalara², David Virieux³, Jean-Luc Pirat³, Yves-Alain Bekro¹

Affiliations

¹ Laboratoire de Chimie Bioorganique et de Substances Naturelles (LCBOSN), UFR-SFA, Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d'Ivoire.
² UMR 5253, ICG Montpellier, Equipe AM2N, ENSCM, 8, rue de l'Ecole Normale, 34296 Montpellier cedex 5, France. ; INSERM U-1051, Institut des Neurosciences de Montpellier, 80 rue Augustin Fliche 34091 Montpellier, France.
³ INSERM U-1051, Institut des Neurosciences de Montpellier, 80 rue Augustin Fliche 34091 Montpellier, France.

Abstract

The n-butanol extract of the roots of Glyphaea brevis was analysed. HPLC analysis suggested the presence of phenolic compounds like protocatechuic acid (PCA). The extract showed moderate cytotoxic activity against C6 glioma cells (EC50 > 1 mg/ml).

Keywords: C6 Cells; Cytotoxicity; Glyphaea brevis; HPLC; Protocatechuic acid.