Characterization of bioactive chitosan and sulfated chitosan from Doryteuthis singhalensis (Ortmann, 1891)

Int J Biol Macromol. 2017 Jun:99:682-691. doi: 10.1016/j.ijbiomac.2017.03.041. Epub 2017 Mar 8.

Abstract

Chitosan was extracted from the pen of squid Doryteuthis singhalensis and characterized using FT-IR, NMR, CHN, SEM and DSC analysis. Purified chitosan was sulfated with chlorosulfonic acid in N,N-dimethylformamide and the added sulfate group was confirmed with FT-IR analysis. The molecular weight and degree of deacetylation (DDA) of chitosan was found 226.6kDa and 83.76% respectively. Chitosan exhibited potent antioxidant activity evidenced by reducing power, chelating ability on ferrous ions and scavenging activity on DPPH, superoxide and hydroxyl radicals. The anticoagulant assay using activated partial thromboplastin time (APTT) and prothrombin time (PT) showed chitosan as a strong anticoagulant. The results of this study showed possibility of using D. singhalensis pen as a non-conventional source of natural antioxidants and anticoagulant which can be incorporated in functional food formulations.

Keywords: Anticoagulant; Antioxidant; Chitosan; Deacetylation; Scavenging ability; Squid.

MeSH terms

  • Acetylation
  • Animals
  • Anticoagulants / chemistry*
  • Anticoagulants / isolation & purification
  • Anticoagulants / pharmacology*
  • Antioxidants / chemistry*
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Chitosan / chemistry*
  • Chitosan / isolation & purification
  • Chitosan / pharmacology*
  • Decapodiformes / chemistry*
  • Free Radicals / chemistry
  • Humans
  • Iron / chemistry
  • Molecular Weight

Substances

  • Anticoagulants
  • Antioxidants
  • Free Radicals
  • chitosan sulfate
  • Chitosan
  • Iron