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3D Cell Culture Models of Epithelial Tissues.
Zhang K, Manninen A. Zhang K, et al. Methods Mol Biol. 2019;1926:77-84. doi: 10.1007/978-1-4939-9021-4_7. Methods Mol Biol. 2019. PMID: 30742264
Here we describe two protocols for culturing epithelial cells in 3D substrates, the "blob culture" setup where cells are fully embedded into BME gel and the "overlay setup" where cells are seeded on top of BME gel and then overlaid with a thin layer of BME (Debnath J, Brugge …
Here we describe two protocols for culturing epithelial cells in 3D substrates, the "blob culture" setup where cells are fully embedded into …