Thermus thermophilus strains active in purine nucleoside synthesis

Marcos Almendros; José-Vicente Sinisterra Gago; José Berenguer Carlos

doi:10.3390/molecules14031279

Thermus thermophilus strains active in purine nucleoside synthesis

Molecules. 2009 Mar 24;14(3):1279-87. doi: 10.3390/molecules14031279.

Authors

Marcos Almendros¹, José-Vicente Sinisterra Gago, José Berenguer Carlos

Affiliation

¹ Biotransformations Group, Servicio de Biotransformaciones Industriales, Parque Científico de Madrid, C/ Santiago Grisolia n masculine 2, 28760 Tres Cantos, Madrid, Spain.

Abstract

Several strains of Thermus thermophilus were tested in order to detect purine nucleoside synthase activity using pyrimidine nucleosides as the sugar-donor and adenine or hypoxanthine as bases. High productivity values (t =1 hr) were obtained while completely avoiding adenosine-deaminase degradation of the products. N-2-deoxy-ribosyltransferase activity is described for the first time in hyperthermophilic bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenine / metabolism
Hypoxanthine / metabolism
Kinetics
Pentosyltransferases
Purine Nucleosides / biosynthesis*
Pyrimidine Nucleosides / metabolism
Thermus thermophilus / enzymology
Thermus thermophilus / metabolism*

Substances

Purine Nucleosides
Pyrimidine Nucleosides
Hypoxanthine
Pentosyltransferases
nucleoside deoxyribosyltransferase II
Adenine