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Detection of tyrosine phosphorylated proteins by combination of immunoaffinity enrichment, two-dimensional difference gel electrophoresis and fluorescent Western blotting.
Bergström Lind S, Hagner-McWhirter S, Elfineh L, Molin M, Jorsback A, Ohman J, Pettersson U. Bergström Lind S, et al. Among authors: ohman j. Biochem Biophys Res Commun. 2010 Oct 29;401(4):581-5. doi: 10.1016/j.bbrc.2010.09.104. Epub 2010 Oct 1. Biochem Biophys Res Commun. 2010. PMID: 20888324
Production of a truncated soluble human semicarbazide-sensitive amine oxidase mediated by a GST-fusion protein secreted from HEK293 cells.
Ohman J, Jakobsson E, Källström U, Elmblad A, Ansari A, Kalderén C, Robertson E, Danielsson E, Gustavsson AL, Varadi A, Ekblom J, Holmgren E, Doverskog M, Abrahmsén L, Nilsson J. Ohman J, et al. Protein Expr Purif. 2006 Apr;46(2):321-31. doi: 10.1016/j.pep.2005.10.027. Epub 2005 Nov 17. Protein Expr Purif. 2006. PMID: 16325418