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Quantitative mapping of the cellular small RNA landscape with AQRNA-seq.
Hu JF, Yim D, Ma D, Huber SM, Davis N, Bacusmo JM, Vermeulen S, Zhou J, Begley TJ, DeMott MS, Levine SS, de Crécy-Lagard V, Dedon PC, Cao B. Hu JF, et al. Among authors: bacusmo jm. Nat Biotechnol. 2021 Aug;39(8):978-988. doi: 10.1038/s41587-021-00874-y. Epub 2021 Apr 15. Nat Biotechnol. 2021. PMID: 33859402 Free PMC article.
Essentiality of threonylcarbamoyladenosine (t(6)A), a universal tRNA modification, in bacteria.
Thiaville PC, El Yacoubi B, Köhrer C, Thiaville JJ, Deutsch C, Iwata-Reuyl D, Bacusmo JM, Armengaud J, Bessho Y, Wetzel C, Cao X, Limbach PA, RajBhandary UL, de Crécy-Lagard V. Thiaville PC, et al. Among authors: bacusmo jm. Mol Microbiol. 2015 Dec;98(6):1199-221. doi: 10.1111/mmi.13209. Epub 2015 Oct 7. Mol Microbiol. 2015. PMID: 26337258 Free PMC article.
The t6A modification acts as a positive determinant for the anticodon nuclease PrrC, and is distinctively nonessential in Streptococcus mutans.
Bacusmo JM, Orsini SS, Hu J, DeMott M, Thiaville PC, Elfarash A, Paulines MJ, Rojas-Benítez D, Meineke B, Deutsch C, Iwata-Reuyl D, Limbach PA, Dedon PC, Rice KC, Shuman S, Crécy-Lagard V. Bacusmo JM, et al. RNA Biol. 2018;15(4-5):508-517. doi: 10.1080/15476286.2017.1353861. Epub 2017 Sep 13. RNA Biol. 2018. PMID: 28726545 Free PMC article.