Changes in bioactive proteins and serum proteome of human milk under different frozen storage

Jingyan Qu; Lina Zhang; Li'ang Yin; Jun Liu; Zhaona Sun; Peng Zhou

doi:10.1016/j.foodchem.2021.129436

Changes in bioactive proteins and serum proteome of human milk under different frozen storage

Food Chem. 2021 Aug 1:352:129436. doi: 10.1016/j.foodchem.2021.129436. Epub 2021 Mar 3.

Authors

Jingyan Qu¹, Lina Zhang², Li'ang Yin³, Jun Liu⁴, Zhaona Sun⁴, Peng Zhou⁵

Affiliations

¹ State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China.
² State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China; School of Food Science & Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China. Electronic address: zhanglina@jiangnan.edu.cn.
³ Haier Smart Technol R&D Co Ltd, 1 Haier Rd, Qingdao 266101, China.
⁴ The Affiliated Wuxi Matemity and Child Health Care Hospital of Nanjing Medical University, China.
⁵ State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China; School of Food Science & Technology, Jiangnan University, Wuxi, Jiangsu Province 214122, China. Electronic address: zhoupeng@jiangnan.edu.cn.

PMID: 33691214
DOI: 10.1016/j.foodchem.2021.129436

Abstract

This study aimed to investigate changes in macronutrients, total bacterial count, and serum proteome of human milk (HM) under different frozen storage (-18°C and -60°C, 60 d and 180 d) by using IBT Labeling proteomics techniques and ELISA kit. The results indicated that total protein concentrations and total aerobic bacterial counts were significantly decreased at -18°C, while no difference at -60°C. A total of 1617 proteins were identified and quantified, and 173 proteins were significantly different. The -18°C storage had much higher influence on HM serum protein profiles than that of -60°C. Increased milk fat globule membrane (MFGM) proteins at -18°C are highly related to the damage of MFGM and transfer of MFGM proteins. The reduction of bioactive proteins is probably related to the ice-induced denaturation. In conclusion, fast cooling and ultra-low constant temperature are more suitable for the cryopreservation of human milk.

Keywords: Bioactive proteins; Frozen storage; Human milk; Proteome; Total bacterial count.

MeSH terms

Animals
Food Storage*
Freezing*
Humans
Milk Proteins / analysis*
Milk, Human / chemistry*
Proteome / chemistry*
Serum / chemistry*

Substances

Milk Proteins
Proteome