Developmental validation of the PowerPlex(®) Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards

Kathryn Oostdik; Kristy Lenz; Jeffrey Nye; Kristin Schelling; Donald Yet; Scott Bruski; Joshua Strong; Clint Buchanan; Joel Sutton; Jessica Linner; Nicole Frazier; Hays Young; Learden Matthies; Amber Sage; Jeff Hahn; Regina Wells; Natasha Williams; Monica Price; Jody Koehler; Melisa Staples; Katie L Swango; Carolyn Hill; Karen Oyerly; Wendy Duke; Lesley Katzilierakis; Martin G Ensenberger; Jeanne M Bourdeau; Cynthia J Sprecher; Benjamin Krenke; Douglas R Storts

doi:10.1016/j.fsigen.2014.04.013

Developmental validation of the PowerPlex(®) Fusion System for analysis of casework and reference samples: A 24-locus multiplex for new database standards

Forensic Sci Int Genet. 2014 Sep:12:69-76. doi: 10.1016/j.fsigen.2014.04.013. Epub 2014 May 14.

Authors

Kathryn Oostdik¹, Kristy Lenz², Jeffrey Nye³, Kristin Schelling⁴, Donald Yet⁵, Scott Bruski⁶, Joshua Strong⁷, Clint Buchanan⁸, Joel Sutton⁹, Jessica Linner¹⁰, Nicole Frazier¹¹, Hays Young¹², Learden Matthies¹³, Amber Sage¹⁴, Jeff Hahn¹⁵, Regina Wells¹⁶, Natasha Williams¹⁷, Monica Price¹⁸, Jody Koehler¹⁹, Melisa Staples²⁰, Katie L Swango²¹, Carolyn Hill²², Karen Oyerly²³, Wendy Duke²⁴, Lesley Katzilierakis²⁵, Martin G Ensenberger²⁶, Jeanne M Bourdeau²⁷, Cynthia J Sprecher²⁸, Benjamin Krenke²⁹, Douglas R Storts³⁰

Affiliations

¹ Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: katie.oostdik@promega.com.
² Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: kristy.lenz@promega.com.
³ Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: NyeJ1@michigan.gov.
⁴ Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: schellingk@michigan.gov.
⁵ Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: yetd@michigan.gov.
⁶ Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: bruskis@michigan.gov.
⁷ Michigan State Police, 333 South Grand Avenue, P.O. Box 30634, Lansing, MI 48909, USA. Electronic address: strongj@michigan.gov.
⁸ United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: clint.d.buchanan.civ@mail.mil.
⁹ United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: joel.d.sutton2.civ@mail.mil.
¹⁰ United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: jessica.k.linner.civ@mail.mil.
¹¹ United States Army Criminal Investigation Laboratory, 4930 North 31st Street, Forest Park, GA 30297, USA(1). Electronic address: nicole.r.frazier.civ@mail.mil.
¹² Arkansas State Crime Laboratory, 3 Natural Resources Drive, Little Rock, AR 72205, USA. Electronic address: hays.young@crimelab.arkansas.gov.
¹³ Los Angeles County Sheriff's Department, 1800 Paseo Rancho Castilla, Los Angeles, CA 90032, USA. Electronic address: LKMatthi@lasd.org.
¹⁴ Los Angeles County Sheriff's Department, 1800 Paseo Rancho Castilla, Los Angeles, CA 90032, USA. Electronic address: ARSage@lasd.org.
¹⁵ Kansas Bureau of Investigation, Forensic Laboratory Division, 1620 Southwest Tyler Street, Topeka, KS 66612, USA. Electronic address: jeff.hahn@kbi.state.ks.us.
¹⁶ Kentucky State Police, 100 Sower Boulevard, Frankfort, KY 40601, USA. Electronic address: regina.wells@ky.gov.
¹⁷ Washington State Patrol, Crime Laboratory Division, 2203 Airport Way South, Seattle, WA 98134-2028, USA. Electronic address: Natasha.williams@wsp.wa.gov.
¹⁸ Washington State Patrol, Crime Laboratory Division, 2203 Airport Way South, Seattle, WA 98134-2028, USA. Electronic address: monica.price@wsp.wa.gov.
¹⁹ Texas Department of Public Safety, Crime Laboratory, 5805 North Lamar Boulevard, Austin, TX 78752, USA. Electronic address: jody.koehler@dps.texas.gov.
²⁰ New Hampshire State Police, Forensic Laboratory, 33 Hazen Drive, Concord, NH 03305, USA. Electronic address: melisa.staples@dos.nh.gov.
²¹ New Hampshire State Police, Forensic Laboratory, 33 Hazen Drive, Concord, NH 03305, USA. Electronic address: katie.swango@dos.nh.gov.
²² National Institute of Standards and Technology, Biochemical Science Division, 100 Bureau Drive, Gaithersburg, MA 20899, USA. Electronic address: becky.hill@nist.gov.
²³ Oklahoma State Bureau of Investigation, 800 East 2nd Street, Edmond, OK 73034, USA. Electronic address: karen.oyerly@osbi.ok.gov.
²⁴ Oklahoma State Bureau of Investigation, 800 East 2nd Street, Edmond, OK 73034, USA. Electronic address: wendy.duke@osbi.ok.gov.
²⁵ Oklahoma State Bureau of Investigation, 800 East 2nd Street, Edmond, OK 73034, USA. Electronic address: Lesley.katzilierakis@osbi.ok.gov.
²⁶ Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: martin.ensenberger@promega.com.
²⁷ Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: jeanne.bourdeau@promega.com.
²⁸ Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: benjamin.krenke@promega.com.
²⁹ Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: cindy.sprecher@promega.com.
³⁰ Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. Electronic address: doug.storts@promega.com.

PMID: 24905335
DOI: 10.1016/j.fsigen.2014.04.013

Abstract

The original CODIS database based on 13 core STR loci has been overwhelmingly successful for matching suspects with evidence. Yet there remain situations that argue for inclusion of more loci and increased discrimination. The PowerPlex(®) Fusion System allows simultaneous amplification of the following loci: Amelogenin, D3S1358, D1S1656, D2S441, D10S1248, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, TH01, vWA, D21S11, D7S820, D5S818, TPOX, DYS391, D8S1179, D12S391, D19S433, FGA, and D22S1045. The comprehensive list of loci amplified by the system generates a profile compatible with databases based on either the expanded CODIS or European Standard Set (ESS) requirements. Developmental validation testing followed SWGDAM guidelines and demonstrated the quality and robustness of the PowerPlex(®) Fusion System across a number of variables. Consistent and high-quality results were compiled using data from 12 separate forensic and research laboratories. The results verify that the PowerPlex(®) Fusion System is a robust and reliable STR-typing multiplex suitable for human identification.

Keywords: Forensic science; PowerPlex(R); Short tandem repeat; Validation.

Publication types

Validation Study

MeSH terms

Databases, Genetic*
Forensic Genetics*
Humans
Microsatellite Repeats