Cytotoxicity of set polymer nanocomposite resin root-end filling materials

Int Endod J. 2011 Feb;44(2):154-61. doi: 10.1111/j.1365-2591.2010.01825.x. Epub 2010 Nov 17.

Abstract

Aim: To evaluate the cytotoxicity of two forms of the novel root-end filling materials, polymer nanocomposite (PNC) resins [C-18 Amine montmorillonate (MMT) and VODAC MMT] both containing Chlorhexidine Diacetate Salt Hydrate 2%, and to compare it to that of two widely accepted commercially available materials, ProRoot® MTA and Geristore®.

Methodology: Elutes of experimental materials extracted after 24 h, 1, 2 and 3 weeks were interacted with the mouse fibroblasts L-929 using a colorimetric cell viability assay (MTS) based on mitochondrial dehydrogenases activity. Using 100% and 50% concentrations of the extracted elutes of the experimental materials the effect of different concentrations of elutes on the cells was analysed. In the positive control group Hygrogold® was added to the cell culture to arrest cells bioactivity. In the negative control group, fresh Dulbecco's Eagle's minimum essential medium supplemented with 10% foetal bovine serum was used to enhance cell bioactivity. Differences in mean bioactivity values were assessed using a t-test and one-way anova (P<0.05).

Results: No significant difference was found in cytotoxicity between ProRoot® MTA, Geristore® and PNC resin C-18 Amine MMT on 24 h, 1, 2 and 3 weeks samples. Sample elutes of PNC resin VODAC MMT, however, revealed cytotoxic activity during most of these experiments.

Conclusion: Cytotoxicity of the elutes of PNC resin C-18 Amine MMT was not significantly different from that of ProRoot® and Geristore®. PNC resin VODAC MMT, revealed significantly more cytotoxicity compared to the other tested materials.

Publication types

  • Comparative Study

MeSH terms

  • Aluminum Compounds / chemistry
  • Aluminum Compounds / toxicity
  • Analysis of Variance
  • Animals
  • Bentonite / chemistry
  • Bentonite / toxicity
  • Calcium Compounds / chemistry
  • Calcium Compounds / toxicity
  • Cell Survival
  • Cells, Cultured
  • Chlorhexidine / chemistry
  • Composite Resins / chemistry
  • Composite Resins / toxicity*
  • Dental Cements / chemistry
  • Dental Cements / toxicity*
  • Drug Combinations
  • Fibroblasts / drug effects*
  • Fibroblasts / enzymology
  • Glass Ionomer Cements / chemistry
  • Glass Ionomer Cements / toxicity
  • Mice
  • Nanocomposites / chemistry
  • Nanocomposites / toxicity*
  • Oxides / chemistry
  • Oxides / toxicity
  • Oxidoreductases / drug effects
  • Polymers / chemistry
  • Polymers / toxicity
  • Quaternary Ammonium Compounds / chemistry
  • Quaternary Ammonium Compounds / toxicity
  • Resins, Synthetic / chemistry
  • Resins, Synthetic / toxicity
  • Root Canal Filling Materials / chemistry
  • Root Canal Filling Materials / toxicity*
  • Silicates / chemistry
  • Silicates / toxicity
  • Statistics, Nonparametric
  • Time Factors
  • Toxicity Tests, Chronic / methods

Substances

  • Aluminum Compounds
  • Calcium Compounds
  • Composite Resins
  • Dental Cements
  • Drug Combinations
  • Geristore
  • Glass Ionomer Cements
  • Oxides
  • Polymers
  • Quaternary Ammonium Compounds
  • Resins, Synthetic
  • Root Canal Filling Materials
  • Silicates
  • mineral trioxide aggregate
  • Bentonite
  • Oxidoreductases
  • Chlorhexidine