Aim: To construct underexpression HSP90alpha and overexpression HSP90beta human hepatoma cell line HepG2.
Methods: The combined plasimid pSilencerHSP90alpha and pSmycHSP90beta were introduced into HepG2 by electroporation, respectively. The result of transfection was identified by Western-blotting and the curve of cell growth was drew by MTT. Observe the cell vitality and expression of HSP90.
Results: Expression of HSP90 in transfected cell line was shown by Western-blotting: Compared with control, expression of HSP90 in the cells transfected with pSilencerHSP90alpha decreased, whereas that in the cells transfected with pSmycHSP90beta increased.The growth curves of the two groups of transfected cells was as the same as that of the control group.
Conclusion: The stable overexpression HSP90beta and underexpression HSP90alpha HepG2 cell lines were established.