Mexican Propolis: A Source of Antioxidants and Anti-Inflammatory Compounds, and Isolation of a Novel Chalcone and ε-Caprolactone Derivative

Molecules. 2018 Feb 6;23(2):334. doi: 10.3390/molecules23020334.

Abstract

The propolis produced by bees are used in alternative medicine for treating inflammation, and infections, presumably due to its antioxidant properties. In this context, five propolis from México were investigated to determine their inhibitory lipid peroxidation properties. The ethyl acetate extract from a red propolis from Chiapas State (4-EAEP) was the most potent (IC50 = 1.42 ± 0.07 μg/mL) in the TBARS assay, and selected for further studies. This extract afforded two new compounds, epoxypinocembrin chalcone (6), and an ε-caprolactone derivative (10), as well as pinostrobin (1), izalpinin (2), cinnamic acid (3), pinocembrin (4), kaempherol (5), 3,3-dimethylallyl caffeate in mixture with isopent-3-enyl caffeate (7a + 7b), 3,4-dimethoxycinnamic acid (8), rhamnetin (9) and caffeic acid (11). The HPLC profile, anti-mycobacterial, and antioxidant properties of this extract was also determined. Most of the isolated compounds were also tested by inhibition of reactive oxygen species (ROS) in challenged mouse bone marrow-derived mast cells (BMMCs), and DPPH. Their anti-inflammatory activity was evaluated by TPA, and MPO (myeloperoxidase) activity by ear edema test in mice. The most potent compounds were 7a + 7b in the TBARS assay (IC50 = 0.49 ± 0.06 μM), and 2 which restored the ROS baseline (3.5 μM). Our results indicate that 4-EAEP has anti-oxidant, and anti-inflammatory properties due to its active compounds, suggesting it has anti-allergy and anti-asthma potential.

Keywords: Mexican propolis; anti-inflammatory activity; antioxidant activity; epoxypinocembrin chalcone; red propolis; ε-lactone.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Anti-Inflammatory Agents / chemistry*
  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants / chemistry*
  • Antioxidants / pharmacology*
  • Caproates / chemistry*
  • Cell Degranulation / drug effects
  • Cell Degranulation / immunology
  • Chalcones / chemistry*
  • Chlorocebus aethiops
  • Chromatography, High Pressure Liquid
  • Lactones / chemistry*
  • Magnetic Resonance Spectroscopy
  • Mast Cells / drug effects
  • Mast Cells / immunology
  • Mast Cells / metabolism
  • Mexico
  • Mice
  • Molecular Structure
  • Peroxidase / antagonists & inhibitors
  • Peroxidase / metabolism
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Propolis / chemistry*
  • Propolis / metabolism
  • Reactive Oxygen Species
  • Spectrometry, Mass, Electrospray Ionization
  • Vero Cells

Substances

  • Anti-Bacterial Agents
  • Anti-Inflammatory Agents
  • Antioxidants
  • Caproates
  • Chalcones
  • Lactones
  • Plant Extracts
  • Reactive Oxygen Species
  • caprolactone
  • Propolis
  • Peroxidase