Cordyceps militaris Reduces Oxidative Stress and Regulates Immune T Cells to Inhibit Metastatic Melanoma Invasion

Yuan-Hong Lan; Yun-Sheng Lu; Ju-Yu Wu; Hsu-Tung Lee; Penjit Srinophakun; Gizem Naz Canko; Chien-Chih Chiu; Hui-Min David Wang

doi:10.3390/antiox11081502

Cordyceps militaris Reduces Oxidative Stress and Regulates Immune T Cells to Inhibit Metastatic Melanoma Invasion

Antioxidants (Basel). 2022 Jul 30;11(8):1502. doi: 10.3390/antiox11081502.

Authors

Yuan-Hong Lan¹, Yun-Sheng Lu², Ju-Yu Wu³, Hsu-Tung Lee⁴, Penjit Srinophakun⁵, Gizem Naz Canko⁶, Chien-Chih Chiu⁷, Hui-Min David Wang^{6

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Affiliations

¹ Department of Medical Laboratory Science and Biotechnology, ASIA University, Taichung 413, Taiwan.
² Taiwan Agriculture Research Institute, Council of Agriculture, Taichung 413, Taiwan.
³ Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung 402, Taiwan.
⁴ The Department of Neurological Institute, Taichung Veterans General Hospital, Taichung 402, Taiwan.
⁵ Chemical Engineering Department, Faculty of Engineering, Kasetsart University, 50 Ngamwongwan Rd., Ladyao, Jatujak, Bangkok 10900, Thailand.
⁶ Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
⁷ Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
⁸ Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
⁹ Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan.

Abstract

In this study, the water extract of Cordyceps militaris (Linn.) Link (CM) was used as a functional material to investigate the inhibitory mechanisms on B16F10 and lung metastatic melanoma (LMM) cells. Reducing power, chelating ability, and 2,2-diphenyl-2-picrylhydrazyl (DPPH) assays were applied for antioxidative capacities, and we obtained positive results from the proper concentrations of CM. To examine the ability of CM in melanoma proliferation inhibition and to substantiate the previous outcomes, three cellular experiments were performed via (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT, a tetrazole) assay, cell migration, and invasion evaluation. The addition of CM to the incubation medium increased the number of CD8+ T cells significantly, which improved the immunogenicity. This study showed that CM exhibits various biological capabilities, including antioxidation, anti-tumor, tumor invasion suppression, and T cytotoxic cell activity promotion.

Keywords: B16F10; Cordyceps militaris; T cell; lung metastatic melanoma; tumor microenvironment.

Abstract

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