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Freeze shattering: a simple and effective method for permeabilizing higher plant cell walls.
Wasteneys GO, Willingale-Theune J, Menzel D. Wasteneys GO, et al. J Microsc. 1997 Oct;188(Pt 1):51-61. doi: 10.1046/j.1365-2818.1977.2390796.x. J Microsc. 1997. PMID: 9369020
It replaces the use of wall degrading enzymes or the need to employ laborious sectioning or other mechanical means for providing access of probes to cells. Freeze-shattering retains the integrity of whole tissues and cells surprisingly well and thus is especially useful wh …
It replaces the use of wall degrading enzymes or the need to employ laborious sectioning or other mechanical means for providing access of p …
Immunohematopoietic stem cell transplantation: introduction and 35 years of development in South Africa--the historical and scientific perspective.
Jacobs P, Wood L. Jacobs P, et al. Bone Marrow Transplant. 2008 Aug;42 Suppl 1:S125-S132. doi: 10.1038/bmt.2008.140. Bone Marrow Transplant. 2008. PMID: 18724285 Review.
This was shortly followed by creating a dedicated platelet donor panel and establishing a specialized laboratory for clonogenic assays, flow cytometry, and programmed freezing and by including cryopreservation. Development was constant and seamless but four distinct period …
This was shortly followed by creating a dedicated platelet donor panel and establishing a specialized laboratory for clonogenic assays, flow …
Vacuum-Induced Surface Freezing to Produce Monoliths of Aligned Porous Alumina.
Großberger S, Fey T, Lee G. Großberger S, et al. Materials (Basel). 2016 Dec 5;9(12):983. doi: 10.3390/ma9120983. Materials (Basel). 2016. PMID: 28774102 Free PMC article.
Vacuum-induced surface freezing has been used to produce uni-directional freezing of colloidal aluminum oxide dispersions. ...The domains are randomly oriented to one another, as already reported for regular freeze casting. This technique for directional f
Vacuum-induced surface freezing has been used to produce uni-directional freezing of colloidal aluminum oxide dispersions. ... …
Can spray freeze-drying improve the re-dispersion of crystalline nanoparticles of pure naproxen?
Braig V, Konnerth C, Peukert W, Lee G. Braig V, et al. Int J Pharm. 2019 Jun 10;564:293-298. doi: 10.1016/j.ijpharm.2019.04.061. Epub 2019 Apr 24. Int J Pharm. 2019. PMID: 31022500
Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose and stabilized with hydroxypropyl cellulose. ...The disaggregation of the SFD nanoparticles was superior to that reported in the literature …
Spray freeze drying (SFD) was used to prepare re-dispersible powders of crystalline, pure-drug nanodispersions of naproxen in lactose …
Shrinkage of spray-freeze-dried microparticles of pure protein for ballistic injection by manipulation of freeze-drying cycle.
Straller G, Lee G. Straller G, et al. Int J Pharm. 2017 Oct 30;532(1):444-449. doi: 10.1016/j.ijpharm.2017.09.036. Epub 2017 Sep 18. Int J Pharm. 2017. PMID: 28923767
Spray-freeze-drying was used to produce shrivelled, partially-collapsed microparticles of pure proteins that may be suitable for use in a ballistic injector. Various modifications of the freeze drying cycle were examined for their effects on collapse of the pure pro …
Spray-freeze-drying was used to produce shrivelled, partially-collapsed microparticles of pure proteins that may be suitable for use …
Tortuosity of Aligned Channels in Alumina Membranes Produced by Vacuum-Induced Surface Directional Freezing.
Großberger S, Fey T, Lee G. Großberger S, et al. Materials (Basel). 2017 Apr 14;10(4):409. doi: 10.3390/ma10040409. Materials (Basel). 2017. PMID: 28772769 Free PMC article.
Vacuum-induced surface freezing of colloidal alumina was used to produce membranes that have elongated, aligned channels and, hence, are tortuous in the direction perpendicular to ice crystal growth. ...
Vacuum-induced surface freezing of colloidal alumina was used to produce membranes that have elongated, aligned channels and, hence, …
Measurement of shrinkage and cracking in lyophilized amorphous cakes. Part IV: Effects of freezing protocol.
Ullrich S, Seyferth S, Lee G. Ullrich S, et al. Int J Pharm. 2015 Nov 10;495(1):52-57. doi: 10.1016/j.ijpharm.2015.08.091. Epub 2015 Sep 2. Int J Pharm. 2015. PMID: 26325321
The shrinkage and cracking of pure trehalose cakes during lyophilization has been determined quantitatively using different protocols for the freezing step. The influences of shelf cooling rate and of a two-step freezing protocol with holding and annealing phases we …
The shrinkage and cracking of pure trehalose cakes during lyophilization has been determined quantitatively using different protocols for th …
Development of cryopelletization and formulation measures to improve stability of Echis carinatus venum protein for use in diagnostic rotational thromboelastometry.
Erber M, Lee G. Erber M, et al. Int J Pharm. 2015 Nov 30;495(2):692-700. doi: 10.1016/j.ijpharm.2015.09.038. Epub 2015 Sep 21. Int J Pharm. 2015. PMID: 26392247
When kept at room temperature the ecarin activity falls to 90% of its initial value after just 39 min. The stability kinetics of ecarin during freezing and lyophilization are strongly dependent on the presence of a stabilizer comprising a collagen peptide-fraction. ...
When kept at room temperature the ecarin activity falls to 90% of its initial value after just 39 min. The stability kinetics of ecarin duri …