Determination of the Qualitative Composition of Biologically-Active Substances of Extracts of In Vitro Callus, Cell Suspension, and Root Cultures of the Medicinal Plant Rhodiola rosea

Biomolecules. 2021 Feb 27;11(3):365. doi: 10.3390/biom11030365.

Abstract

The results of the qualitative composition analysis of the dried biomass extracts of in vitro callus, cell suspension, and root cultures show that the main biologically active substances (BAS) in the medicinal plant, Rhodiola rosea, are 6-C-(1-(4-hydroxyphenyl)ethyl)aromadendrin (25 mg, yield 0.21%), 2-(3,7-dihydroxy-2-(2-hydroxypropan-2-yl)-2,3-dihydrobenzofuran-5-yl)-6,7-dihydroxychroman-4-one (23 mg, yield 0.2%), 2-(3,4-dimethoxyphenyl)-5,7-dimethoxychroman-4-one (175 mg, yield 1.5%), 5,7-dihydroxy-2-(4-hydroxy-3-(2-(4-hydroxyphenyl)-4-oxo-4H-chromen-6-yl)phenyl)-4H-chromen-4-one (45 mg, yield 0.5%), 5,6,7,8-tetrahydroxy-4-methoxyflavone (0.35 mg, 0.5%). BAS from the dried biomass extracts of in vitro callus, cell suspension, and root cultures of Rhodiola rosea will be used for the production of pharmaceuticals and dietary supplements with antitumor, antimicrobial, and antioxidant effects.

Keywords: 1H NMR spectra; HPLC; Rhodiola rosea; biologically-active substances; cell suspension; in vitro callus; root cultures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Flavonoids / analysis
  • Flavonoids / chemistry
  • Plant Cells / chemistry*
  • Plant Extracts / chemistry*
  • Plants, Medicinal / chemistry*
  • Proton Magnetic Resonance Spectroscopy
  • Rhodiola / chemistry*

Substances

  • Flavonoids
  • Plant Extracts